Structure of the E. COLI trigger factor bound to a translating ribosome

Structure

electron microscopy · 4 distinct chains · 0% helix, 0% strand (DSSP).

Ligands and binding sites

• MSE · Selenomethionine · ion · chains A

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Interfaces

2 interfaces between chains.

• A–B · 59 Ų buried · 0 hydrogen bonds · protein-protein
• B–D · 45 Ų buried · 0 hydrogen bonds · protein-protein

Modifications

Observed in the structure: 10 modified (engineered).

Compared structures

• 2VRH · Structure of the E. coli trigger factor bound to a translating ribosome · the same entry in another collection · chain A against A, TM-score 1.00
• P0A850 · AlphaFold monomer V2.0 prediction for trigger factor (P0A850) · a predicted model of the same protein · chain A against A, TM-score 0.86
• Q0TCE3 · AlphaFold monomer V2.0 prediction for 50S ribosomal protein L23 (Q0TCE3) · a predicted model of the same protein · chain B against A, TM-score 0.81
• P60624 · AlphaFold monomer V2.0 prediction for 50S ribosomal protein L24 (P60624) · a predicted model of the same protein · chain C against A, TM-score 0.71
• P0A7M6 · AlphaFold monomer V2.0 prediction for 50S ribosomal protein L29 (P0A7M6) · a predicted model of the same protein · chain D against A, TM-score 0.65

Analyse

• Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
• Every structure of UniProt P0A850
• Every structure of UniProt Q0TCE3
• Every structure of UniProt P60624
• Every structure of UniProt P0A7M6
• Elsewhere: RCSB PDB 2VRH · PDBe 2VRH · UniProt P0A850 · UniProt Q0TCE3 · UniProt P60624 · UniProt P0A7M6