Research workbench
Human erythrocyte pyruvate kinase: T384M mutant
Analyse Human erythrocyte pyruvate kinase: T384M mutant in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 2VGF
- Type
- protein
- Length
- 2,037 residues
- Chains
- 4
- Method
- x-ray diffraction
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Pyruvate kinase isozymes R/L
- EC
- 2.7.1.40
- UniProt
- P30613
- Ligands
- FBP, PGA, K, MN
- Pockets
- 20 · best druggability 0.86
Structure
x-ray diffraction · 3 distinct chains · 41% helix, 21% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A, C | Pyruvate kinase isozymes R/L (EC 2.7.1.40) | protein | 517 | 40% helix, 21% strand | P30613 |
| B | Pyruvate kinase isozymes R/L (EC 2.7.1.40) | protein | 491 | 42% helix, 20% strand | P30613 |
| D | Pyruvate kinase isozymes R/L (EC 2.7.1.40) | protein | 512 | 41% helix, 21% strand | P30613 |
Ligands and binding sites
- FBP · 1,6-di-O-phosphono-beta-D-fructofuranose · ligand · 4 copies · chains A, B, C, D
- PGA · 2-phosphoglycolic acid · ligand · 4 copies · chains A, B, C, D
- K · Potassium ion · metal · 4 copies · chains A, B, C, D
- MN · Manganese (II) ion · metal · 4 copies · chains A, B, C, D
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
20 pockets found on a 1 Å grid, 18 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 322 | 0.86 | 0.96 | 42 | empty |
| 2 | 321 | 0.85 | 0.95 | 42 | empty |
| 3 | 577 | 0.82 | 0.88 | 46 | empty |
| 4 | 609 | 0.80 | 0.85 | 67 | empty |
| 5 | 464 | 0.78 | 0.86 | 53 | empty |
Interfaces
6 interfaces between chains.
- A–C · 3011 Ų buried · 33 hydrogen bonds · protein-protein
- B–D · 2982 Ų buried · 41 hydrogen bonds · protein-protein
- C–D · 1541 Ų buried · 31 hydrogen bonds · protein-protein
- A–B · 1516 Ų buried · 28 hydrogen bonds · protein-protein
- A–D · 37 Ų buried · 0 hydrogen bonds · protein-protein
Compared structures
- 2VGF · HUMAN ERYTHROCYTE PYRUVATE KINASE: T384M mutant · the same entry in another collection · chain A against A, TM-score 1.00
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P30613
- Elsewhere: RCSB PDB 2VGF · PDBe 2VGF · UniProt P30613
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