Research workbench

Mouse nicotinamide N-methyltransferase

Analyse Mouse nicotinamide N-methyltransferase in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.

PDB: 2I62
Type: protein
Length: 1,035 residues
Chains: 4
Method: x-ray diffraction
Collection: MolNFT v1 / PDB
Contract: 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
Owner: 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
Molecule: Nicotinamide N-methyltransferase
EC: 2.1.1.1
UniProt: O55239
Ligands: SAH
Pockets: 8 · best druggability 0.81

Structure

x-ray diffraction · 4 distinct chains · 38% helix, 25% strand (DSSP).

Chain	Molecule	Type	Residues	Secondary structure	UniProt
A	Nicotinamide N-methyltransferase (EC 2.1.1.1)	protein	257	39% helix, 25% strand	O55239
B	Nicotinamide N-methyltransferase (EC 2.1.1.1)	protein	261	38% helix, 25% strand	O55239
C	Nicotinamide N-methyltransferase (EC 2.1.1.1)	protein	258	39% helix, 25% strand	O55239
D	Nicotinamide N-methyltransferase (EC 2.1.1.1)	protein	259	38% helix, 25% strand	O55239

Ligands and binding sites

SAH · S-adenosyl-L-homocysteine · ligand · 4 copies · chains A, B, C, D

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Pockets

8 pockets found on a 1 Å grid, 8 with a druggability of 0.5 or more.

Pocket	Volume (Å³)	Druggability	Buriedness	Lining residues	Inside
1	211	0.81	0.94	43	SAH
2	196	0.80	0.94	40	SAH
3	202	0.80	0.94	45	SAH
4	230	0.79	0.92	44	SAH
5	681	0.72	0.79	96	empty

Interfaces

4 interfaces between chains.

C–D · 604 Å² buried · 11 hydrogen bonds · protein-protein
A–B · 580 Å² buried · 6 hydrogen bonds · protein-protein
B–C · 570 Å² buried · 14 hydrogen bonds · protein-protein
A–C · 466 Å² buried · 4 hydrogen bonds · protein-protein

Compared structures

2I62 · Mouse Nicotinamide N-methyltransferase · the same entry in another collection · chain D against D, TM-score 1.00
O55239 · AlphaFold monomer V2.0 prediction for nicotinamide N-methyltransferase (O55239) · a predicted model of the same protein · chain A against A, TM-score 0.98

Analyse

Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
Every structure of UniProt O55239
Elsewhere: RCSB PDB 2I62 · PDBe 2I62 · UniProt O55239

GL1X is a live explorer of the GenesisL1 blockchain. Turn on JavaScript for search, charts and interactive views.