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Crystal structure of E.COLI RNase hi active site mutant (K87A/H124A)

Analyse Crystal structure of E.COLI RNase hi active site mutant (K87A/H124A) in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.

PDB: 1WSJ
Type: protein
Length: 1,210 residues
Chains: 8
Method: x-ray diffraction
Collection: MolNFT v1 / PDB
Contract: 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
Owner: 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
Molecule: Ribonuclease hi
EC: 3.1.26.4
UniProt: P0A7Y4
Pockets: 9 · best druggability 0.44

Structure

x-ray diffraction · 7 distinct chains · 36% helix, 32% strand (DSSP).

Chain	Molecule	Type	Residues	Secondary structure	UniProt
A	Ribonuclease hi (EC 3.1.26.4)	protein	155	35% helix, 31% strand	P0A7Y4
B, E	Ribonuclease hi (EC 3.1.26.4)	protein	153	36% helix, 31% strand	P0A7Y4
C	Ribonuclease hi (EC 3.1.26.4)	protein	149	38% helix, 31% strand	P0A7Y4
D	Ribonuclease hi (EC 3.1.26.4)	protein	149	37% helix, 32% strand	P0A7Y4
F	Ribonuclease hi (EC 3.1.26.4)	protein	152	35% helix, 32% strand	P0A7Y4
G	Ribonuclease hi (EC 3.1.26.4)	protein	148	38% helix, 34% strand	P0A7Y4
H	Ribonuclease hi (EC 3.1.26.4)	protein	151	35% helix, 33% strand	P0A7Y4

Pockets

9 pockets found on a 1 Å grid, 0 with a druggability of 0.5 or more.

Pocket	Volume (Å³)	Druggability	Buriedness	Lining residues	Inside
1	64	0.44	0.82	14	empty
2	193	0.44	0.73	26	empty
3	57	0.40	0.81	13	empty
4	58	0.36	0.79	13	empty
5	76	0.36	0.76	13	empty

Interfaces

3 interfaces between chains.

B–E · 135 Å² buried · 2 hydrogen bonds · protein-protein
C–F · 59 Å² buried · 0 hydrogen bonds · protein-protein
A–H · 47 Å² buried · 0 hydrogen bonds · protein-protein

Compared structures

1WSJ · Crystal structure of E.coli RNase HI active site mutant (K87A/H124A) · the same entry in another collection · chain G against G, TM-score 1.00
P0A7Y4 · AlphaFold monomer V2.0 prediction for ribonuclease hi (P0A7Y4) · a predicted model of the same protein · chain C against A, TM-score 0.99

Analyse

Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
Every structure of UniProt P0A7Y4
Elsewhere: RCSB PDB 1WSJ · PDBe 1WSJ · UniProt P0A7Y4

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