Crystallographic and solution studies of N-lithocholyl insulin: a new generation of prolonged-acting insulins.

Structure

x-ray diffraction · 2 distinct chains · 67% helix, 4% strand (DSSP).

Ligands and binding sites

• CRS · M-cresol · ligand · chains A, B
• UZ9 · (2S)-2-amino-6-({(4R)-4-[(10R,13S)-10,13-dimethyl-3-oxohexadecahydro-1H-cyclopenta[a]phenanthren-17-yl]pentanoyl}amino)hexanoic acid · ligand · chains A, B
• ZN · Zinc ion · metal · chains B
• CL · Chloride ion · ion · chains B

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Interfaces

1 interface between chains.

• A–B · 595 Ų buried · 6 hydrogen bonds · protein-protein

Modifications

Observed in the structure: 3 disulfide, 1 covalent (disulfide, covalent ligand).

Compared structures

• 1UZ9 · Crystallographic and solution studies of N-lithocholyl insulin: a new generation of… · the same entry in another collection · chain A against A, TM-score 1.00
• P01308 · AlphaFold monomer V2.0 prediction for insulin (P01308) · a predicted model of the same protein · chain B against A, TM-score 0.50

Analyse

• Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
• Every structure of UniProt P01308
• Elsewhere: RCSB PDB 1UZ9 · PDBe 1UZ9 · UniProt P01308