Research workbench
Structural mechanisms of camptothecin resistance by mutations in human topoisomerase I
Analyse Structural mechanisms of camptothecin resistance by mutations in human… in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 1RR8
- Type
- protein + DNA
- Length
- 538 residues
- Chains
- 3
- Method
- x-ray diffraction
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- 5'-D(*AP*AP*AP*AP*AP*GP*AP*CP*TP*T*GP*GP*AP*AP*AP*AP*AP*TP* TP*TP*TP*T)-3'…
- EC
- 5.99.1.2
- UniProt
- P11387
- Ligands
- TTG, TTC
- Pockets
- 10 · best druggability 0.87
Structure
x-ray diffraction · 3 distinct chains · 42% helix, 14% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | 5'-D(*AP*AP*AP*AP*AP*GP*AP*CP*TP*T*GP*GP*AP*AP*AP*AP*AP*TP* TP*TP*TP*T)-3' | dna | 22 | — | — |
| B | 5'-D(*AP*AP*AP*AP*AP*TP*TP*TP*TP*TP*CP*CP*AP*AP*GP*TP*CP*TP *TP*TP*TP*T)-3' | dna | 22 | — | — |
| C | DNA topoisomerase I (EC 5.99.1.2) | protein | 494 | 42% helix, 14% strand | P11387 |
Ligands and binding sites
- TTG · 2-(1-dimethylaminomethyl-2-hydroxy-8-hydroxymethyl-9-oxo-9,11-dihydro-indolizino[1,2-b]quinolin-7-yl)-2-hydroxy-butyric acid · ligand · chains A, B, C
- TTC · (S)-10-[(dimethylamino)methyl]-4-ethyl-4,9-dihydroxy-1H-pyrano[3',4':6,7]inolizino[1,2-B]-quinoline-3, 14(4H,12H)-dione · ligand · chains A, B, C
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
10 pockets found on a 1 Å grid, 2 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 1146 | 0.87 | 0.88 | 116 | TTG, TTC |
| 2 | 114 | 0.63 | 0.88 | 30 | empty |
| 3 | 62 | 0.49 | 0.85 | 14 | empty |
| 4 | 77 | 0.48 | 0.82 | 10 | empty |
| 5 | 68 | 0.44 | 0.81 | 13 | empty |
Interfaces
3 interfaces between chains.
- A–B · 993 Ų buried · 70 hydrogen bonds · nucleic-nucleic
- A–C · 971 Ų buried · 16 hydrogen bonds · protein-nucleic
- B–C · 824 Ų buried · 22 hydrogen bonds · protein-nucleic
Modifications
Observed in the structure: 1 modified (phosphorylation).
Compared structures
- 1RR8 · Structural Mechanisms of Camptothecin Resistance by Mutations in Human Topoisomerase I · the same entry in another collection · chain A against A, TM-score 1.00
- P11387 · AlphaFold monomer V2.0 prediction for DNA topoisomerase 1 (P11387) · a predicted model of the same protein · chain C against A, TM-score 0.99
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P11387
- Elsewhere: RCSB PDB 1RR8 · PDBe 1RR8 · UniProt P11387
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