Crystal structure of barley alpha-amylase isozyme 1 (AMY1) inactive mutant D180A in complex with acarbose

Structure

x-ray diffraction · 1 distinct chain · 32% helix, 19% strand (DSSP).

Ligands and binding sites

• DAF · 4,6-dideoxy-4-{[(1S,5R,6S)-3-formyl-5,6-dihydroxy-4-oxocyclohex-2-en-1-yl]amino}-alpha-D-xylo-hex-5-enopyranose · ligand · 3 copies · chains A
• GLC · alpha-D-glucopyranose · sugar · 5 copies · chains A
• BGC · beta-D-glucopyranose · sugar · chains A
• CA · Calcium ion · metal · 3 copies · chains A

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Pockets

1 pocket found on a 1 Å grid, 0 with a druggability of 0.5 or more.

Compared structures

• 1RP9 · Crystal structure of barley alpha-amylase isozyme 1 (amy1) inactive mutant d180a in… · the same entry in another collection · chain A against A, TM-score 1.00
• P00693 · AlphaFold monomer V2.0 prediction for alpha-amylase type a isozyme (P00693) · a predicted model of the same protein · chain A against A, TM-score 1.00

Analyse

• Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
• Every structure of UniProt P00693
• Elsewhere: RCSB PDB 1RP9 · PDBe 1RP9 · UniProt P00693