Research workbench
Protein engineering of xylose (glucose) isomerase from actinoplanes missouriensis. 1. crystallography and site- directed mutagenesis of metal binding sites
Analyse Protein engineering of xylose (glucose) isomerase from actinoplanes… in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 7XIM
- Type
- protein
- Length
- 1,560 residues
- Chains
- 4
- Method
- x-ray diffraction
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- D-xylose isomerase
- EC
- 5.3.1.5
- UniProt
- P12851
- Pockets
- 14 · best druggability 0.84
Structure
x-ray diffraction · 1 distinct chain · 49% helix, 10% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A, B, C, D | D-xylose isomerase (EC 5.3.1.5) | protein | 390 | 49% helix, 10% strand | P12851 |
Pockets
14 pockets found on a 1 Å grid, 10 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 809 | 0.84 | 0.86 | 103 | empty |
| 2 | 802 | 0.83 | 0.86 | 99 | empty |
| 3 | 549 | 0.79 | 0.85 | 59 | empty |
| 4 | 571 | 0.79 | 0.85 | 58 | empty |
| 5 | 557 | 0.79 | 0.85 | 58 | empty |
Interfaces
6 interfaces between chains.
- A–C · 5027 Ų buried · 82 hydrogen bonds · protein-protein
- B–D · 4996 Ų buried · 84 hydrogen bonds · protein-protein
- C–D · 1767 Ų buried · 22 hydrogen bonds · protein-protein
- A–B · 1722 Ų buried · 29 hydrogen bonds · protein-protein
- B–C · 1680 Ų buried · 23 hydrogen bonds · protein-protein
Compared structures
- 7XIM · Protein engineering of xylose (glucose) isomerase from actinoplanes missouriensis. 1.… · the same entry in another collection · chain A against A, TM-score 1.00
- P12851 · AlphaFold monomer V2.0 prediction for xylose isomerase (P12851) · a predicted model of the same protein · chain A against A, TM-score 1.00
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P12851
- Elsewhere: RCSB PDB 7XIM · PDBe 7XIM · UniProt P12851
GL1X is a live explorer of the GenesisL1 blockchain. Turn on JavaScript for search, charts and interactive views.