Research workbench
The curved structure of MPIEZO1 in lipid bilayer
Analyse The curved structure of MPIEZO1 in lipid bilayer in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 7WLT
- Type
- protein
- Length
- 4,059 residues
- Chains
- 3
- Method
- electron microscopy
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Piezo-type mechanosensitive ion channel component 1
- UniProt
- E2JF22
- Ligands
- PLX, PEE, P5S
- Pockets
- 20 · best druggability 0.85
Structure
electron microscopy · 1 distinct chain · 61% helix, 7% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A, C, E | Piezo-type mechanosensitive ion channel component 1 | protein | 1353 | 61% helix, 7% strand | E2JF22 |
Ligands and binding sites
- PLX · (9R,11S)-9-({[(1S)-1-hydroxyhexadecyl]oxy}methyl)-2,2-dimethyl-5,7,10-trioxa-2lambda~5~-aza-6lambda~5~-phosphaoctacosane-6,6,11-triol · ligand · 21 copies · chains A, C, E
- PEE · 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine · ligand · 3 copies · chains A, E, C
- P5S · O-[(R)-{[(2R)-2,3-bis(octadecanoyloxy) propyl]oxy}(hydroxy)phosphoryl]-L-serine · ligand · 3 copies · chains A, C, E
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
20 pockets found on a 1 Å grid, 20 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 602 | 0.85 | 0.90 | 50 | empty |
| 2 | 948 | 0.81 | 0.82 | 79 | PLX |
| 3 | 893 | 0.81 | 0.82 | 81 | PEE |
| 4 | 951 | 0.81 | 0.82 | 91 | PEE |
| 5 | 960 | 0.81 | 0.82 | 77 | PLX |
Interfaces
3 interfaces between chains.
- C–E · 3620 Ų buried · 27 hydrogen bonds · protein-protein
- A–E · 3616 Ų buried · 27 hydrogen bonds · protein-protein
- A–C · 3614 Ų buried · 27 hydrogen bonds · protein-protein
Modifications
Observed in the structure: 3 disulfide (disulfide).
Compared structures
- 7WLT · the Curved Structure of mPIEZO1 in Lipid Bilayer · the same entry in another collection · chain A against A, TM-score 1.00
- E2JF22 · AlphaFold monomer V2.0 prediction for piezo-type mechanosensitive ion channel component 1… · a predicted model of the same protein · chain A against A, TM-score 0.81
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt E2JF22
- Elsewhere: RCSB PDB 7WLT · PDBe 7WLT · UniProt E2JF22
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