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Cryo-EM structure of E.COLI ftsh-hflkc aaa protease complex

Analyse Cryo-EM structure of E.COLI ftsh-hflkc aaa protease complex in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.

PDB: 7WI3
Type: protein
Length: 8,688 residues
Chains: 48
Method: electron microscopy
Collection: MolNFT v1 / PDB
Contract: 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
Owner: 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
Molecule: Modulator of ftsh protease hflc; Modulator of ftsh protease hflk; ATP-dependent zinc…
EC: 3.4.24.-
UniProt: P0ABC3, P0ABC7, P0AAI3
Pockets: 20 · best druggability 0.84

Structure

electron microscopy · 7 distinct chains · 48% helix, 16% strand (DSSP).

Chain	Molecule	Type	Residues	Secondary structure	UniProt
A, Y, Z, M	Modulator of ftsh protease hflc	protein	293	51% helix, 12% strand	P0ABC3
B, F, G, a, i, k, b, j, l, N, R, S	Modulator of ftsh protease hflk	protein	275	53% helix, 17% strand	P0ABC7
C, c, d, O	Modulator of ftsh protease hflc	protein	287	52% helix, 15% strand	P0ABC3
D, e, f, P	Modulator of ftsh protease hflc	protein	290	51% helix, 13% strand	P0ABC3
E, H, g, m, h, n, Q, T	ATP-dependent zinc metalloprotease ftsh (EC 3.4.24.-)	protein	81	30% helix, 28% strand	P0AAI3
I, K, L, o, s, u, p, t, v, U, W, X	ATP-dependent zinc metalloprotease ftsh (EC 3.4.24.-)	protein	79	29% helix, 27% strand	P0AAI3
J, q, r, V	ATP-dependent zinc metalloprotease ftsh (EC 3.4.24.-)	protein	78	33% helix, 24% strand	P0AAI3

Pockets

20 pockets found on a 1 Å grid, 20 with a druggability of 0.5 or more.

Pocket	Volume (Å³)	Druggability	Buriedness	Lining residues	Inside
1	640	0.84	0.89	107	empty
2	973	0.81	0.82	106	empty
3	452	0.81	0.89	85	empty
4	962	0.81	0.82	96	empty
5	1190	0.80	0.81	102	empty

Interfaces

120 interfaces between chains.

Z–b · 4377 Å² buried · 19 hydrogen bonds · protein-protein
M–N · 4288 Å² buried · 20 hydrogen bonds · protein-protein
Y–a · 4268 Å² buried · 15 hydrogen bonds · protein-protein
A–B · 4257 Å² buried · 21 hydrogen bonds · protein-protein
D–G · 4253 Å² buried · 37 hydrogen bonds · protein-protein

Compared structures

7WI3 · Cryo-EM structure of E.Coli FtsH-HflkC AAA protease complex · the same entry in another collection · chain K against K, TM-score 1.00

Analyse

Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
Every structure of UniProt P0ABC3
Every structure of UniProt P0ABC7
Every structure of UniProt P0AAI3
Elsewhere: RCSB PDB 7WI3 · PDBe 7WI3 · UniProt P0ABC3 · UniProt P0ABC7 · UniProt P0AAI3

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