Research workbench
Cryoem structure of the pancreatic ATP-sensitive potassium channel bound to ATP with KIR6.2-ctd in the down conformation
Analyse Cryoem structure of the pancreatic ATP-sensitive potassium channel bound to ATP… in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 7U1E
- Type
- protein
- Length
- 2,741 residues
- Chains
- 5
- Method
- electron microscopy
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- ATP-sensitive inward rectifier potassium channel 11; ATP-binding cassette sub-family C…
- UniProt
- P70673, Q09427
- Ligands
- ATP, NAG
- Pockets
- 20 · best druggability 0.88
Structure
electron microscopy · 4 distinct chains · 52% helix, 12% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | ATP-sensitive inward rectifier potassium channel 11 | protein | 326 | 34% helix, 21% strand | P70673 |
| B | ATP-sensitive inward rectifier potassium channel 11 | protein | 327 | 31% helix, 23% strand | P70673 |
| C, D | ATP-sensitive inward rectifier potassium channel 11 | protein | 328 | 30% helix, 23% strand | P70673 |
| E | ATP-binding cassette sub-family C member 8 | protein | 1432 | 65% helix, 5% strand | Q09427 |
Ligands and binding sites
- ATP · Adenosine-5'-triphosphate · ligand · 5 copies · chains A, B, C, D, E
- NAG · 2-acetamido-2-deoxy-beta-D-glucopyranose · sugar · chains E
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
20 pockets found on a 1 Å grid, 20 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 913 | 0.88 | 0.90 | 137 | empty |
| 2 | 1004 | 0.88 | 0.89 | 131 | empty |
| 3 | 639 | 0.86 | 0.90 | 96 | empty |
| 4 | 489 | 0.84 | 0.91 | 66 | empty |
| 5 | 1053 | 0.84 | 0.85 | 133 | empty |
Interfaces
8 interfaces between chains.
- A–D · 2584 Ų buried · 10 hydrogen bonds · protein-protein
- B–C · 2570 Ų buried · 17 hydrogen bonds · protein-protein
- C–D · 2556 Ų buried · 14 hydrogen bonds · protein-protein
- A–B · 2407 Ų buried · 14 hydrogen bonds · protein-protein
- A–E · 1289 Ų buried · 2 hydrogen bonds · protein-protein
Modifications
Observed in the structure: 5 disulfide, 1 glycan (disulfide, N-glycosylation).
Compared structures
- 7U1E · CryoEM structure of the pancreatic ATP-sensitive potassium channel bound to ATP with… · the same entry in another collection · chain E against E, TM-score 1.00
- Q09427 · AlphaFold monomer V2.0 prediction for ATP-binding cassette sub-family C member 8 (Q09427) · a predicted model of the same protein · chain E against A, TM-score 0.98
- P70673 · AlphaFold monomer V2.0 prediction for ATP-sensitive inward rectifier potassium channel 11… · a predicted model of the same protein · chain C against A, TM-score 0.89
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P70673
- Every structure of UniProt Q09427
- Elsewhere: RCSB PDB 7U1E · PDBe 7U1E · UniProt P70673 · UniProt Q09427
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