Research workbench

T-plastin-F-actin complex

Analyse T-plastin-F-actin complex in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.

PDB: 7R94
Type: protein
Length: 2,570 residues
Chains: 8
Method: electron microscopy
Collection: MolNFT v1 / PDB
Contract: 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
Owner: 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
Molecule: Actin, alpha skeletal muscle; Plastin-3
UniProt: P68139, P13797
Ligands: ADP, MG
Pockets: 20 · best druggability 0.80

Structure

electron microscopy · 2 distinct chains · 48% helix, 12% strand (DSSP).

Chain	Molecule	Type	Residues	Secondary structure	UniProt
C, B, A, D, E	Actin, alpha skeletal muscle	protein	370	44% helix, 20% strand	P68139
F, G, H	Plastin-3	protein	240	53% helix, 0% strand	P13797

Ligands and binding sites

ADP · Adenosine-5'-diphosphate · ligand · 5 copies · chains C, B, A, D, E
MG · Magnesium ion · metal · 5 copies · chains C, B, A, D, E

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Pockets

20 pockets found on a 1 Å grid, 15 with a druggability of 0.5 or more.

Pocket	Volume (Å³)	Druggability	Buriedness	Lining residues	Inside
1	1133	0.80	0.81	85	empty
2	703	0.78	0.83	76	empty
3	304	0.77	0.88	52	ADP, MG
4	312	0.76	0.88	49	ADP, MG
5	292	0.76	0.88	52	ADP, MG

Interfaces

13 interfaces between chains.

C–A · 1207 Å² buried · 10 hydrogen bonds · protein-protein
C–E · 1198 Å² buried · 10 hydrogen bonds · protein-protein
B–D · 1197 Å² buried · 10 hydrogen bonds · protein-protein
B–H · 892 Å² buried · 9 hydrogen bonds · protein-protein
C–G · 890 Å² buried · 8 hydrogen bonds · protein-protein

Modifications

Observed in the structure: 5 modified, 3 covalent (methylation, covalent ligand).

Compared structures

7R94 · T-Plastin-F-actin complex · the same entry in another collection · chain H against H, TM-score 1.00
P68139 · AlphaFold monomer V2.0 prediction for actin, alpha skeletal muscle (P68139) · a predicted model of the same protein · chain D against A, TM-score 0.96

Analyse

Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
Every structure of UniProt P68139
Every structure of UniProt P13797
Elsewhere: RCSB PDB 7R94 · PDBe 7R94 · UniProt P68139 · UniProt P13797

GL1X is a live explorer of the GenesisL1 blockchain. Turn on JavaScript for search, charts and interactive views.