Research workbench
Cryo-EM MAP of human telomerase-DNA-TPP1 complex (sharpened)
Analyse Cryo-EM MAP of human telomerase-DNA-TPP1 complex (sharpened) in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 7QXA
- Type
- protein + DNA + RNA
- Length
- 1,512 residues
- Chains
- 6
- Method
- electron microscopy
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Telomerase reverse transcriptase; Human telomerase RNA; Histone H2A; Histone H2B…
- EC
- 2.7.7.49
- UniProt
- O14746, B2R5B3, B4DR52, A0A590TQL1
- Pockets
- 20 · best druggability 0.79
Structure
electron microscopy · 6 distinct chains · 47% helix, 14% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | Telomerase reverse transcriptase (EC 2.7.7.49) | protein | 954 | 47% helix, 15% strand | O14746 |
| B | Human telomerase RNA | rna | 253 | — | — |
| L | Histone H2A | protein | 81 | 62% helix, 0% strand | B2R5B3 |
| M | Histone H2B | protein | 90 | 73% helix, 0% strand | B4DR52 |
| N | Telomeric DNA | dna | 12 | — | — |
| O | Adrenocortical dysplasia homolog (mouse), isoform CRA_A | protein | 122 | 16% helix, 30% strand | A0A590TQL1 |
Pockets
20 pockets found on a 1 Å grid, 20 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 1031 | 0.79 | 0.81 | 55 | empty |
| 2 | 779 | 0.77 | 0.82 | 51 | empty |
| 3 | 385 | 0.76 | 0.86 | 48 | empty |
| 4 | 712 | 0.76 | 0.81 | 57 | empty |
| 5 | 340 | 0.75 | 0.87 | 26 | empty |
Interfaces
8 interfaces between chains.
- A–B · 6568 Ų buried · 119 hydrogen bonds · protein-nucleic
- L–M · 2553 Ų buried · 16 hydrogen bonds · protein-protein
- A–N · 1389 Ų buried · 15 hydrogen bonds · protein-nucleic
- A–O · 1016 Ų buried · 10 hydrogen bonds · protein-protein
- B–L · 550 Ų buried · 10 hydrogen bonds · protein-nucleic
Compared structures
- 7QXA · Cryo-EM map of human telomerase-DNA-TPP1 complex (sharpened) · the same entry in another collection · chain L against L, TM-score 1.00
- O14746 · AlphaFold monomer V2.0 prediction for telomerase reverse transcriptase (O14746) · a predicted model of the same protein · chain A against A, TM-score 0.92
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt O14746
- Every structure of UniProt B2R5B3
- Every structure of UniProt B4DR52
- Every structure of UniProt A0A590TQL1
- Elsewhere: RCSB PDB 7QXA · PDBe 7QXA · UniProt O14746 · UniProt B2R5B3 · UniProt B4DR52 · UniProt A0A590TQL1
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