Research workbench
HIV-1 reverse transcriptase complex with DNA and inhibitor rmc-247
Analyse HIV-1 reverse transcriptase complex with DNA and inhibitor rmc-247 in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 7OTN
- Type
- protein + DNA
- Length
- 2,021 residues
- Chains
- 8
- Method
- x-ray diffraction
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Reverse TRANSCRIPTASE/RIBONUCLEASE H; DNA (5'…
- EC
- 2.7.7.49, 2.7.7.7, 3.1.26.13, 3.1.13.2
- UniProt
- P03366
- Ligands
- 1IH, MN
- Pockets
- 20 · best druggability 0.85
Structure
x-ray diffraction · 7 distinct chains · 33% helix, 22% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| C | Reverse TRANSCRIPTASE/RIBONUCLEASE H (EC 2.7.7.49, 2.7.7.7, 3.1.26.13, 3.1.13.2) | protein | 555 | 31% helix, 23% strand | P03366 |
| D | Reverse TRANSCRIPTASE/RIBONUCLEASE H (EC 2.7.7.49, 2.7.7.7, 3.1.26.13, 3.1.13.2) | protein | 411 | 36% helix, 19% strand | P03366 |
| A | Reverse TRANSCRIPTASE/RIBONUCLEASE H (EC 2.7.7.49, 2.7.7.7, 3.1.26.13, 3.1.13.2) | protein | 556 | 30% helix, 23% strand | P03366 |
| B | Reverse TRANSCRIPTASE/RIBONUCLEASE H (EC 2.7.7.49, 2.7.7.7, 3.1.26.13, 3.1.13.2) | protein | 412 | 36% helix, 19% strand | P03366 |
| E | DNA (5'- D(P*GP*GP*TP*CP*GP*GP*CP*GP*CP*CP*CP*GP*AP*AP*CP*AP*GP*GP*GP*AP*CP*TP *G)-3') | dna | 23 | — | — |
| F, P | DNA (5'-D(*CP*AP*GP*TP*CP*CP*CP*TP*GP*TP*TP*CP*GP*GP*(MRG) P*CP*GP*CP*CP*(DDG))-3') | dna | 20 | — | — |
| T | DNA (5'- D(P*GP*GP*TP*CP*GP*GP*CP*GP*CP*CP*CP*GP*AP*AP*CP*AP*GP*GP*GP*AP*CP*TP *G)-3') | dna | 24 | — | — |
Ligands and binding sites
- 1IH · (S)-2-((2-(6-amino-9H-purin-9-yl)ethyl)amino)-3-phosphonopropanoic acid · ligand · 2 copies · chains C, E, F, A, T, P
- MN · Manganese (II) ion · metal · 2 copies · chains C, A
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
20 pockets found on a 1 Å grid, 19 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 829 | 0.85 | 0.88 | 79 | empty |
| 2 | 474 | 0.84 | 0.91 | 61 | empty |
| 3 | 288 | 0.80 | 0.92 | 32 | empty |
| 4 | 578 | 0.80 | 0.85 | 50 | empty |
| 5 | 456 | 0.78 | 0.86 | 40 | empty |
Interfaces
13 interfaces between chains.
- C–D · 2950 Ų buried · 36 hydrogen bonds · protein-protein
- A–B · 2899 Ų buried · 30 hydrogen bonds · protein-protein
- A–T · 1383 Ų buried · 15 hydrogen bonds · protein-nucleic
- C–E · 1246 Ų buried · 9 hydrogen bonds · protein-nucleic
- C–F · 992 Ų buried · 15 hydrogen bonds · protein-nucleic
Modifications
Observed in the structure: 4 modified (nucleotide modification).
Compared structures
- 7OTN · HIV-1 reverse transcriptase complex with DNA and inhibitor rmc-247 · the same entry in another collection · chain C against C, TM-score 1.00
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P03366
- Elsewhere: RCSB PDB 7OTN · PDBe 7OTN · UniProt P03366
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