Research workbench
Crystal structure of a mutant ihf (BETAE44A) complexed with the native H' site
Analyse Crystal structure of a mutant ihf (BETAE44A) complexed with the native H' site in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 1OWF
- Type
- protein + DNA
- Length
- 260 residues
- Chains
- 5
- Method
- x-ray diffraction
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Phage lambda H' site; 5'-D(*GP*GP*CP*CP*AP*AP*AP*AP*AP*AP*GP*CP*AP*TP*T)-3'…
- UniProt
- P0A6X7, P0A6Y1
- Pockets
- 5 · best druggability 0.79
Structure
x-ray diffraction · 5 distinct chains · 41% helix, 29% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| C | Phage lambda H' site | dna | 35 | — | — |
| D | 5'-D(*GP*GP*CP*CP*AP*AP*AP*AP*AP*AP*GP*CP*AP*TP*T)-3' | dna | 15 | — | — |
| E | 5'-D(*GP*CP*TP*TP*AP*TP*CP*AP*AP*TP*TP*TP*GP*TP*TP*GP*CP*AP *CP*C)-3' | dna | 20 | — | — |
| A | Integration host factor alpha-subunit | protein | 96 | 42% helix, 29% strand | P0A6X7 |
| B | Integration host factor beta-subunit | protein | 94 | 40% helix, 30% strand | P0A6Y1 |
Pockets
5 pockets found on a 1 Å grid, 3 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 783 | 0.79 | 0.83 | 73 | empty |
| 2 | 313 | 0.62 | 0.80 | 25 | empty |
| 3 | 331 | 0.56 | 0.75 | 31 | empty |
| 4 | 157 | 0.44 | 0.75 | 16 | empty |
| 5 | 110 | 0.37 | 0.75 | 16 | empty |
Interfaces
10 interfaces between chains.
- A–B · 2542 Ų buried · 26 hydrogen bonds · protein-protein
- C–E · 927 Ų buried · 70 hydrogen bonds · nucleic-nucleic
- C–B · 787 Ų buried · 17 hydrogen bonds · protein-nucleic
- C–D · 692 Ų buried · 50 hydrogen bonds · nucleic-nucleic
- C–A · 672 Ų buried · 11 hydrogen bonds · protein-nucleic
Compared structures
- 1OWF · Crystal structure of a mutant IHF (BetaE44A) complexed with the native H' Site · the same entry in another collection · chain D against D, TM-score 1.00
- P0A6Y1 · AlphaFold monomer V2.0 prediction for integration host factor subunit beta (P0A6Y1) · a predicted model of the same protein · chain B against A, TM-score 0.99
- P0A6X7 · AlphaFold monomer V2.0 prediction for integration host factor subunit alpha (P0A6X7) · a predicted model of the same protein · chain A against A, TM-score 0.96
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P0A6X7
- Every structure of UniProt P0A6Y1
- Elsewhere: RCSB PDB 1OWF · PDBe 1OWF · UniProt P0A6X7 · UniProt P0A6Y1
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