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Crystal structure of 14-3-3 epsilon with 9J10 peptide

Analyse Crystal structure of 14-3-3 epsilon with 9J10 peptide in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.

PDB: 7C8E
Type: protein
Length: 473 residues
Chains: 4
Method: x-ray diffraction
Collection: MolNFT v1 / PDB
Contract: 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
Owner: 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
Molecule: 14-3-3 protein epsilon; 9J10
UniProt: P62258
Pockets: 6 · best druggability 0.54

Structure

x-ray diffraction · 3 distinct chains · 78% helix, 0% strand (DSSP).

Chain	Molecule	Type	Residues	Secondary structure	UniProt
A, B	14-3-3 protein epsilon	protein	230	82% helix, 0% strand	P62258
C	9J10	protein	6	0% helix, 0% strand	—
D	9J10	protein	7	0% helix, 0% strand	—

Ligands and binding sites

TRS · 2-amino-2-hydroxymethyl-propane-1,3-diol · additive · chains B

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Pockets

6 pockets found on a 1 Å grid, 2 with a druggability of 0.5 or more.

Pocket	Volume (Å³)	Druggability	Buriedness	Lining residues	Inside
1	338	0.54	0.74	28	empty
2	128	0.52	0.80	21	empty
3	117	0.45	0.78	19	empty
4	97	0.45	0.79	14	empty
5	89	0.44	0.79	16	empty

Interfaces

3 interfaces between chains.

A–B · 1041 Å² buried · 9 hydrogen bonds · protein-protein
A–C · 587 Å² buried · 18 hydrogen bonds · protein-protein
B–D · 539 Å² buried · 21 hydrogen bonds · protein-protein

Modifications

Observed in the structure: 2 modified (phosphorylation).

Compared structures

7C8E · Crystal Structure of 14-3-3 epsilon with 9J10 peptide · the same entry in another collection · chain A against A, TM-score 1.00
P62258 · AlphaFold monomer V2.0 prediction for 14-3-3 protein epsilon (P62258) · a predicted model of the same protein · chain B against A, TM-score 0.97

Analyse

Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
Every structure of UniProt P62258
Elsewhere: RCSB PDB 7C8E · PDBe 7C8E · UniProt P62258

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