Research workbench
Type I-F CRISPR-csy complex with its inhibitor ACRF6
Analyse Type I-F CRISPR-csy complex with its inhibitor ACRF6 in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 6VQX
- Type
- protein + RNA
- Length
- 3,032 residues
- Chains
- 11
- Method
- electron microscopy
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- ACRF6; CRISPR-associated protein CSY1; Type I-F CRISPR-associated protein CSY2…
- EC
- 3.1.-.-
- UniProt
- A0A5D4V3F2, Q02ML9, B3G161, A0A444M080, Q02MM2
- Pockets
- 20 · best druggability 0.83
Structure
electron microscopy · 9 distinct chains · 25% helix, 19% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | ACRF6 | protein | 97 | 57% helix, 0% strand | A0A5D4V3F2 |
| B | CRISPR-associated protein CSY1 | protein | 422 | 36% helix, 9% strand | Q02ML9 |
| C | Type I-F CRISPR-associated protein CSY2 | protein | 305 | 20% helix, 30% strand | B3G161 |
| D | CRISPR-associated protein CSY3 | protein | 293 | 24% helix, 24% strand | A0A444M080 |
| E, H, I | CRISPR-associated protein CSY3 | protein | 333 | 25% helix, 24% strand | A0A444M080 |
| F | CRISPR-associated protein CSY3 | protein | 334 | 25% helix, 23% strand | A0A444M080 |
| G | CRISPR-associated protein CSY3 | protein | 335 | 21% helix, 22% strand | A0A444M080 |
| J | CRISPR-associated endonuclease CAS6/CSY4 (EC 3.1.-.-) | protein | 187 | 3% helix, 6% strand | Q02MM2 |
| K | Crrna (60-mer) | rna | 60 | — | — |
Pockets
20 pockets found on a 1 Å grid, 20 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 930 | 0.83 | 0.84 | 94 | empty |
| 2 | 1049 | 0.81 | 0.83 | 105 | empty |
| 3 | 169 | 0.78 | 0.94 | 29 | empty |
| 4 | 1018 | 0.78 | 0.80 | 82 | empty |
| 5 | 1122 | 0.78 | 0.80 | 122 | empty |
Interfaces
30 interfaces between chains.
- B–C · 4859 Ų buried · 70 hydrogen bonds · protein-protein
- E–F · 2211 Ų buried · 30 hydrogen bonds · protein-protein
- F–G · 2186 Ų buried · 30 hydrogen bonds · protein-protein
- H–I · 2156 Ų buried · 20 hydrogen bonds · protein-protein
- G–H · 2128 Ų buried · 28 hydrogen bonds · protein-protein
Compared structures
- 6VQX · Type I-F CRISPR-Csy complex with its inhibitor AcrF6 · the same entry in another collection · chain K against K, TM-score 1.00
- Q02MM2 · AlphaFold monomer V2.0 prediction for CRISPR-associated endonuclease CAS6/CSY4 (Q02MM2) · a predicted model of the same protein · chain J against A, TM-score 0.72
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt A0A5D4V3F2
- Every structure of UniProt Q02ML9
- Every structure of UniProt B3G161
- Every structure of UniProt A0A444M080
- Every structure of UniProt Q02MM2
- Elsewhere: RCSB PDB 6VQX · PDBe 6VQX · UniProt A0A5D4V3F2 · UniProt Q02ML9 · UniProt B3G161 · UniProt A0A444M080 · UniProt Q02MM2
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