Research workbench
Structure of a truncated adenylyl cyclase bound to mant-GTP, forskolin and an activated stimulatory galphas protein
Analyse Structure of a truncated adenylyl cyclase bound to mant-GTP, forskolin and an… in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 6R4O
- Type
- protein
- Length
- 1,182 residues
- Chains
- 2
- Method
- electron microscopy
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Adenylate cyclase 9; Guanine nucleotide-binding protein G(S) subunit alpha isoforms short
- UniProt
- E1BM79, P04896
- Ligands
- ONM, FOK, GSP, MN, MG
- Pockets
- 20 · best druggability 0.83
Structure
electron microscopy · 2 distinct chains · 53% helix, 12% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | Adenylate cyclase 9 | protein | 841 | 56% helix, 13% strand | E1BM79 |
| B | Guanine nucleotide-binding protein G(S) subunit alpha isoforms short | protein | 341 | 46% helix, 10% strand | P04896 |
Ligands and binding sites
- ONM · 3'-O-(N-methylanthraniloyl)-guanosine-5'-triphosphate · ligand · chains A
- FOK · Forskolin · ligand · chains A
- GSP · 5'-guanosine-diphosphate-monothiophosphate · ligand · chains B
- MN · Manganese (II) ion · metal · 2 copies · chains A
- MG · Magnesium ion · metal · chains B
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
20 pockets found on a 1 Å grid, 15 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 780 | 0.83 | 0.86 | 84 | empty |
| 2 | 644 | 0.79 | 0.84 | 78 | GSP, MG |
| 3 | 576 | 0.76 | 0.82 | 71 | empty |
| 4 | 863 | 0.76 | 0.79 | 83 | empty |
| 5 | 917 | 0.73 | 0.77 | 76 | ONM, FOK, MN |
Interfaces
1 interface between chains.
- A–B · 1120 Ų buried · 6 hydrogen bonds · protein-protein
Compared structures
- 6R4O · Structure of a truncated adenylyl cyclase bound to MANT-GTP, forskolin and an activated… · the same entry in another collection · chain A against A, TM-score 1.00
- P04896 · AlphaFold monomer V2.0 prediction for guanine nucleotide-binding protein G(S) subunit… · a predicted model of the same protein · chain B against A, TM-score 0.97
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt E1BM79
- Every structure of UniProt P04896
- Elsewhere: RCSB PDB 6R4O · PDBe 6R4O · UniProt E1BM79 · UniProt P04896
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