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Cryo-EM structure of csm-crrna binary complex in type III-a CRISPR-CAS system

Analyse Cryo-EM structure of csm-crrna binary complex in type III-a CRISPR-CAS system in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.

PDB
6MUU
Type
protein + RNA
Length
2,108 residues
Chains
7
Method
electron microscopy
Collection
MolNFT v1 / PDB
Contract
0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
Owner
0x3396CDB397284571d12822B4ae1189a8E22AAAAA
UniProt
B6YWB8, B6YWB9, B6YWC0, B6YWC1, B6YWC2
Ligands
ZN

Structure

electron microscopy · 7 distinct chains.

ChainMoleculeTypeResiduesSecondary structureUniProt
A—protein780—B6YWB8
B—protein136—B6YWB9
C—protein276—B6YWC0
D—protein283—B6YWC0
E—protein285—B6YWC1
F—protein323—B6YWC2
G—rna25——

Ligands and binding sites

  • ZN · Zinc ion · metal · 3 copies · chains A, C, D

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Compared structures

  • 6MUU · Cryo-EM structure of Csm-crRNA binary complex in type III-A CRISPR-Cas system · the same entry in another collection · chain G against G, TM-score 1.00
  • B6YWB8 · AlphaFold monomer V2.0 prediction for CRISPR system single-strand- specific… · a predicted model of the same protein · chain A against A, TM-score 0.98
  • B6YWC1 · AlphaFold monomer V2.0 prediction for CRISPR system cms protein CSM4 (B6YWC1) · a predicted model of the same protein · chain E against A, TM-score 0.96

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