Research workbench
Cryoem structure of the MDA5-dsrna filament with 74-degree helical twist
Analyse Cryoem structure of the MDA5-dsrna filament with 74-degree helical twist in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 6G19
- Type
- protein + RNA
- Length
- 710 residues
- Chains
- 3
- Method
- electron microscopy
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Interferon-induced helicase C domain-containing protein 1; RNA…
- EC
- 3.6.4.13
- UniProt
- Q8R5F7
- Ligands
- ANP, ZN
- Pockets
- 11 · best druggability 0.86
Structure
electron microscopy · 3 distinct chains · 45% helix, 13% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | Interferon-induced helicase C domain-containing protein 1 (EC 3.6.4.13) | protein | 682 | 45% helix, 13% strand | Q8R5F7 |
| X | RNA (5'-R(P*CP*AP*AP*GP*CP*CP*GP*AP*GP*GP*AP*GP*AP*G)-3') | rna | 14 | — | — |
| Y | RNA (5'-R(P*CP*UP*CP*UP*CP*CP*UP*CP*GP*GP*CP*UP*UP*G)-3') | rna | 14 | — | — |
Ligands and binding sites
- ANP · Phosphoaminophosphonic acid-adenylate ester · ligand · chains A
- ZN · Zinc ion · metal · chains A
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
11 pockets found on a 1 Å grid, 6 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 689 | 0.86 | 0.90 | 79 | empty |
| 2 | 585 | 0.80 | 0.86 | 58 | empty |
| 3 | 439 | 0.74 | 0.84 | 50 | ANP |
| 4 | 231 | 0.70 | 0.86 | 21 | empty |
| 5 | 116 | 0.67 | 0.90 | 19 | empty |
Interfaces
3 interfaces between chains.
- A–Y · 1180 Ų buried · 21 hydrogen bonds · protein-nucleic
- A–X · 1168 Ų buried · 18 hydrogen bonds · protein-nucleic
- X–Y · 687 Ų buried · 62 hydrogen bonds · nucleic-nucleic
Compared structures
- 6G19 · CryoEM structure of the MDA5-dsRNA filament with 74-degree helical twist · the same entry in another collection · chain A against A, TM-score 1.00
- Q8R5F7 · AlphaFold monomer V2.0 prediction for interferon-induced helicase C domain-containing… · a predicted model of the same protein · chain A against A, TM-score 0.99
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt Q8R5F7
- Elsewhere: RCSB PDB 6G19 · PDBe 6G19 · UniProt Q8R5F7
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