Research workbench
Structure of 14-3-3 zeta in complex with ASK1 14-3-3 binding motif
Analyse Structure of 14-3-3 zeta in complex with ASK1 14-3-3 binding motif in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 6EJL
- Type
- protein
- Length
- 463 residues
- Chains
- 4
- Method
- x-ray diffraction
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- 14-3-3 protein ZETA/DELTA; Mitogen-activated protein kinase kinase kinase 5
- EC
- 2.7.11.25
- UniProt
- P63104, Q99683
- Pockets
- 3 · best druggability 0.59
Structure
x-ray diffraction · 4 distinct chains · 81% helix, 0% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | 14-3-3 protein ZETA/DELTA | protein | 225 | 85% helix, 0% strand | P63104 |
| B | 14-3-3 protein ZETA/DELTA | protein | 225 | 82% helix, 0% strand | P63104 |
| C | Mitogen-activated protein kinase kinase kinase 5 (EC 2.7.11.25) | protein | 6 | 0% helix, 0% strand | Q99683 |
| D | Mitogen-activated protein kinase kinase kinase 5 (EC 2.7.11.25) | protein | 7 | 0% helix, 0% strand | Q99683 |
Pockets
3 pockets found on a 1 Å grid, 2 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 186 | 0.59 | 0.82 | 26 | empty |
| 2 | 317 | 0.55 | 0.75 | 26 | empty |
| 3 | 74 | 0.45 | 0.81 | 18 | empty |
Interfaces
3 interfaces between chains.
- A–B · 1075 Ų buried · 15 hydrogen bonds · protein-protein
- B–D · 569 Ų buried · 14 hydrogen bonds · protein-protein
- A–C · 502 Ų buried · 14 hydrogen bonds · protein-protein
Modifications
Observed in the structure: 2 modified (phosphorylation).
Compared structures
- 6EJL · Structure of 14-3-3 zeta in complex with ASK1 14-3-3 binding motif · the same entry in another collection · chain D against D, TM-score 1.00
- P63104 · AlphaFold monomer V2.0 prediction for 14-3-3 protein ZETA/DELTA (P63104) · a predicted model of the same protein · chain A against A, TM-score 0.99
- Q99683 · AlphaFold monomer V2.0 prediction for mitogen-activated protein kinase kinase kinase 5… · a predicted model of the same protein · chain C against A, TM-score 0.65
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P63104
- Every structure of UniProt Q99683
- Elsewhere: RCSB PDB 6EJL · PDBe 6EJL · UniProt P63104 · UniProt Q99683
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