Research workbench
Kaiso (ZBTB33) zinc finger DNA binding domain in complex with a double cpg-methylated DNA resembling the specific kaiso binding sequence (kbs)
Analyse Kaiso (ZBTB33) zinc finger DNA binding domain in complex with a double… in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 5VMW
- Type
- protein + DNA
- Length
- 157 residues
- Chains
- 3
- Method
- x-ray diffraction
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Transcriptional regulator kaiso; DNA (5'-D(*TP*GP*CP*TP*TP*CP*CP*(5CM)P*GP*(5CM)…
- UniProt
- Q86T24
- Ligands
- ZN, CL
- Pockets
- 5 · best druggability 0.72
Structure
x-ray diffraction · 3 distinct chains · 31% helix, 21% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | Transcriptional regulator kaiso | protein | 121 | 31% helix, 21% strand | Q86T24 |
| D | DNA (5'-D(*TP*GP*CP*TP*TP*CP*CP*(5CM)P*GP*(5CM) P*GP*AP*AP*TP*AP*AP*CP*G)-3') | dna | 18 | — | — |
| E | DNA (5'-D(*CP*GP*TP*TP*AP*TP*TP*(5CM)P*GP*(5CM) P*GP*GP*GP*AP*AP*GP*CP*A)-3') | dna | 18 | — | — |
Ligands and binding sites
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
5 pockets found on a 1 Å grid, 2 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 186 | 0.72 | 0.89 | 23 | empty |
| 2 | 152 | 0.56 | 0.81 | 19 | empty |
| 3 | 85 | 0.38 | 0.77 | 16 | empty |
| 4 | 108 | 0.38 | 0.75 | 18 | empty |
| 5 | 102 | 0.33 | 0.73 | 20 | empty |
Interfaces
3 interfaces between chains.
- D–E · 832 Ų buried · 84 hydrogen bonds · nucleic-nucleic
- A–E · 828 Ų buried · 21 hydrogen bonds · protein-nucleic
- A–D · 810 Ų buried · 15 hydrogen bonds · protein-nucleic
Modifications
Observed in the structure: 4 modified (nucleotide modification).
Compared structures
- 5VMW · Kaiso (ZBTB33) zinc finger DNA binding domain in complex with a double CpG-methylated DNA… · the same entry in another collection · chain A against A, TM-score 1.00
- Q86T24 · AlphaFold monomer V2.0 prediction for transcriptional regulator kaiso (Q86T24) · a predicted model of the same protein · chain A against A, TM-score 0.98
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt Q86T24
- Elsewhere: RCSB PDB 5VMW · PDBe 5VMW · UniProt Q86T24
GL1X is a live explorer of the GenesisL1 blockchain. Turn on JavaScript for search, charts and interactive views.