Crystal structure of the porcine carboxypeptidase B - anabaenopeptin C complex

Structure

x-ray diffraction · 2 distinct chains · 38% helix, 18% strand (DSSP).

Ligands and binding sites

• 73P · (2~{S})-6-azanyl-2-(carboxyamino)hexanoic acid · ligand · 3 copies · chains A, F, B, G, C, H
• 73O · (2~{S})-2-azanyl-4-(4-hydroxyphenyl)butanoic acid · ligand · 3 copies · chains A, F, B, G, C, H
• ZN · Zinc ion · metal · 3 copies · chains A, F, B, G, C, H

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Pockets

4 pockets found on a 1 Å grid, 4 with a druggability of 0.5 or more.

Interfaces

6 interfaces between chains.

• A–C · 532 Ų buried · 2 hydrogen bonds · protein-protein
• C–H · 286 Ų buried · 4 hydrogen bonds · protein-protein
• A–F · 280 Ų buried · 4 hydrogen bonds · protein-protein
• B–G · 274 Ų buried · 4 hydrogen bonds · protein-protein
• B–C · 212 Ų buried · 2 hydrogen bonds · protein-protein

Modifications

Observed in the structure: 9 covalent, 9 disulfide, 3 d-amino, 3 isopeptide, 3 modified (covalent ligand, disulfide, D-amino acid, isopeptide, nonstandard).

Compared structures

• 5LRJ · Crystal structure of the porcine carboxypeptidase B - Anabaenopeptin C complex · the same entry in another collection · chain H against H, TM-score 1.00
• P09955 · AlphaFold monomer V2.0 prediction for carboxypeptidase B (P09955) · a predicted model of the same protein · chain A against A, TM-score 1.00

Analyse

• Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
• Every structure of UniProt P09955
• Elsewhere: RCSB PDB 5LRJ · PDBe 5LRJ · UniProt P09955