Research workbench
Two tandem rrm domains of PUF60 bound to an adml pre-mRNA 3' splice site analogue with a modified binding-site nucleic acid base
Analyse Two tandem rrm domains of PUF60 bound to an adml pre-mRNA 3' splice site… in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 5KW6
- Type
- protein + DNA
- Length
- 403 residues
- Chains
- 3
- Method
- x-ray diffraction
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- POLY(U)-binding-splicing factor PUF60; DNA (30-mer)
- UniProt
- Q9UHX1
- Pockets
- 3 · best druggability 0.72
Structure
x-ray diffraction · 3 distinct chains · 33% helix, 28% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | POLY(U)-binding-splicing factor PUF60 | protein | 201 | 33% helix, 27% strand | Q9UHX1 |
| B | POLY(U)-binding-splicing factor PUF60 | protein | 198 | 33% helix, 28% strand | Q9UHX1 |
| C | DNA (30-mer) | dna | 4 | — | — |
Pockets
3 pockets found on a 1 Å grid, 1 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 976 | 0.72 | 0.76 | 96 | empty |
| 2 | 119 | 0.38 | 0.74 | 24 | empty |
| 3 | 106 | 0.35 | 0.73 | 25 | empty |
Interfaces
3 interfaces between chains.
- A–B · 580 Ų buried · 15 hydrogen bonds · protein-protein
- B–C · 370 Ų buried · 9 hydrogen bonds · protein-nucleic
- A–C · 368 Ų buried · 9 hydrogen bonds · protein-nucleic
Compared structures
- 5KW6 · Two Tandem RRM Domains of PUF60 Bound to an AdML Pre-mRNA 3' Splice Site Analogue with a… · the same entry in another collection · chain A against A, TM-score 1.00
- Q9UHX1 · AlphaFold monomer V2.0 prediction for POLY(U)-binding-splicing factor PUF60 (Q9UHX1) · a predicted model of the same protein · chain B against A, TM-score 0.95
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt Q9UHX1
- Elsewhere: RCSB PDB 5KW6 · PDBe 5KW6 · UniProt Q9UHX1
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