Research workbench
Crystal structure of C-cbl tkbd in complex with SPRY2 peptide (36-60, PY55) refined to 1.8 a resolution (P21 form)
Analyse Crystal structure of C-cbl tkbd in complex with SPRY2 peptide (36-60, PY55)… in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 5HKZ
- Type
- protein
- Length
- 327 residues
- Chains
- 2
- Method
- x-ray diffraction
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- E3 ubiquitin-protein ligase cbl; Protein sprouty homolog 2
- EC
- 6.3.2.-
- UniProt
- P22681, O43597
- Ligands
- ACE, NA
- Pockets
- 4 · best druggability 0.55
Structure
x-ray diffraction · 2 distinct chains · 52% helix, 8% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | E3 ubiquitin-protein ligase cbl (EC 6.3.2.-) | protein | 303 | 55% helix, 8% strand | P22681 |
| B | Protein sprouty homolog 2 | protein | 24 | 13% helix, 0% strand | O43597 |
Ligands and binding sites
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
4 pockets found on a 1 Å grid, 1 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 134 | 0.55 | 0.82 | 18 | empty |
| 2 | 132 | 0.44 | 0.76 | 19 | empty |
| 3 | 54 | 0.38 | 0.80 | 17 | empty |
| 4 | 58 | 0.36 | 0.78 | 16 | empty |
Interfaces
1 interface between chains.
- A–B · 1143 Ų buried · 19 hydrogen bonds · protein-protein
Modifications
Observed in the structure: 1 covalent, 1 modified (covalent ligand, phosphorylation).
Compared structures
- 5HKZ · Crystal Structure of c-Cbl TKBD in complex with SPRY2 peptide (36-60, pY55) Refined to… · the same entry in another collection · chain A against A, TM-score 1.00
- P22681 · AlphaFold monomer V2.0 prediction for E3 ubiquitin-protein ligase cbl (P22681) · a predicted model of the same protein · chain A against A, TM-score 0.98
- O43597 · AlphaFold monomer V2.0 prediction for protein sprouty homolog 2 (O43597) · a predicted model of the same protein · chain B against A, TM-score 0.24
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P22681
- Every structure of UniProt O43597
- Elsewhere: RCSB PDB 5HKZ · PDBe 5HKZ · UniProt P22681 · UniProt O43597
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