Research workbench
Recombinant expression, purification and preliminary crystallographic studies of the mature form of human mitochondrial aspartate aminotransferase
Analyse Recombinant expression, purification and preliminary crystallographic studies… in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 5AX8
- Type
- protein
- Length
- 1,604 residues
- Chains
- 4
- Method
- x-ray diffraction
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Aspartate aminotransferase, mitochondrial
- EC
- 2.6.1.1, 2.6.1.7
- UniProt
- P00505
- Pockets
- 20 · best druggability 0.81
Structure
x-ray diffraction · 1 distinct chain · 48% helix, 15% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A, B, C, D | Aspartate aminotransferase, mitochondrial (EC 2.6.1.1, 2.6.1.7) | protein | 401 | 48% helix, 15% strand | P00505 |
Pockets
20 pockets found on a 1 Å grid, 8 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 1093 | 0.81 | 0.83 | 112 | empty |
| 2 | 603 | 0.73 | 0.80 | 57 | empty |
| 3 | 513 | 0.73 | 0.82 | 54 | empty |
| 4 | 586 | 0.71 | 0.80 | 71 | empty |
| 5 | 228 | 0.68 | 0.85 | 29 | empty |
Interfaces
5 interfaces between chains.
- B–D · 3440 Ų buried · 49 hydrogen bonds · protein-protein
- A–C · 3299 Ų buried · 41 hydrogen bonds · protein-protein
- A–B · 481 Ų buried · 4 hydrogen bonds · protein-protein
- A–D · 320 Ų buried · 0 hydrogen bonds · protein-protein
- C–D · 319 Ų buried · 2 hydrogen bonds · protein-protein
Compared structures
- 5AX8 · Recombinant expression, purification and preliminary crystallographic studies of the… · the same entry in another collection · chain D against D, TM-score 1.00
- P00505 · AlphaFold monomer V2.0 prediction for aspartate aminotransferase, mitochondrial (P00505) · a predicted model of the same protein · chain A against A, TM-score 1.00
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P00505
- Elsewhere: RCSB PDB 5AX8 · PDBe 5AX8 · UniProt P00505
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