Research workbench
Crystal structure of tceo RNase H-a chimera combining the folding core from T. thermophilus RNase H and the remaining region of E. COLI RNase H
Analyse Crystal structure of tceo RNase H-a chimera combining the folding core from T.… in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 1JL2
- Type
- protein
- Length
- 562 residues
- Chains
- 4
- Method
- x-ray diffraction
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Chimera of ribonuclease hi, ribonuclease H
- EC
- 3.1.26.4
- UniProt
- P0A7Y4, P29253
- Pockets
- 6 · best druggability 0.74
Structure
x-ray diffraction · 4 distinct chains · 35% helix, 32% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | Chimera of ribonuclease hi, ribonuclease H (EC 3.1.26.4) | protein | 150 | 36% helix, 31% strand | P0A7Y4, P29253 |
| B | Chimera of ribonuclease hi, ribonuclease H (EC 3.1.26.4) | protein | 133 | 36% helix, 35% strand | P0A7Y4, P29253 |
| C | Chimera of ribonuclease hi, ribonuclease H (EC 3.1.26.4) | protein | 133 | 35% helix, 33% strand | P0A7Y4, P29253 |
| D | Chimera of ribonuclease hi, ribonuclease H (EC 3.1.26.4) | protein | 146 | 32% helix, 32% strand | P0A7Y4, P29253 |
Pockets
6 pockets found on a 1 Å grid, 2 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 1097 | 0.74 | 0.77 | 91 | empty |
| 2 | 447 | 0.67 | 0.79 | 59 | empty |
| 3 | 141 | 0.49 | 0.78 | 22 | empty |
| 4 | 67 | 0.35 | 0.77 | 14 | empty |
| 5 | 66 | 0.32 | 0.76 | 15 | empty |
Interfaces
5 interfaces between chains.
- A–C · 603 Ų buried · 5 hydrogen bonds · protein-protein
- B–D · 517 Ų buried · 7 hydrogen bonds · protein-protein
- B–C · 423 Ų buried · 5 hydrogen bonds · protein-protein
- A–D · 316 Ų buried · 2 hydrogen bonds · protein-protein
- A–B · 189 Ų buried · 0 hydrogen bonds · protein-protein
Compared structures
- 1JL2 · Crystal structure of TCEO RNase H-a chimera combining the folding core from T.… · the same entry in another collection · chain A against A, TM-score 1.00
- P0A7Y4 · AlphaFold monomer V2.0 prediction for ribonuclease hi (P0A7Y4) · a predicted model of the same protein · chain C against A, TM-score 0.97
- P29253 · AlphaFold monomer V2.0 prediction for ribonuclease H (P29253) · a predicted model of the same protein · chain D against A, TM-score 0.93
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P0A7Y4
- Every structure of UniProt P29253
- Elsewhere: RCSB PDB 1JL2 · PDBe 1JL2 · UniProt P0A7Y4 · UniProt P29253
GL1X is a live explorer of the GenesisL1 blockchain. Turn on JavaScript for search, charts and interactive views.