Crystal structure of XEG5A, a GH5 xyloglucan-specific endo-beta-1,4- glucanase from ruminal metagenomic library, in complex with a xyloglucan oligosaccharide and tris

Structure

x-ray diffraction · 2 distinct chains · 42% helix, 14% strand (DSSP).

Ligands and binding sites

• BGC · beta-D-glucopyranose · sugar · 5 copies · chains B, A
• XYS · alpha-D-xylopyranose · sugar · 3 copies · chains B, A
• GAL · beta-D-galactopyranose · sugar · 2 copies · chains B, A
• MG · Magnesium ion · metal · 2 copies · chains B, A
• TRS · 2-amino-2-hydroxymethyl-propane-1,3-diol · additive · 2 copies · chains B, A

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Pockets

3 pockets found on a 1 Å grid, 3 with a druggability of 0.5 or more.

Interfaces

1 interface between chains.

• B–A · 345 Ų buried · 2 hydrogen bonds · protein-protein

Modifications

Observed in the structure: 10 modified (engineered).

Compared structures

• 4W88 · Crystal structure of XEG5A, a GH5 xyloglucan-specific endo-beta-1,4-glucanase from… · the same entry in another collection · chain B against B, TM-score 1.00

Analyse

• Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
• Every structure of UniProt D2K7Z0
• Elsewhere: RCSB PDB 4W88 · PDBe 4W88 · UniProt D2K7Z0