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Conformational changes in cubic insulin crystals in the pH range 7-11

Analyse Conformational changes in cubic insulin crystals in the pH range 7-11 in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.

PDB: 1APH
Type: protein
Length: 51 residues
Chains: 2
Method: x-ray diffraction
Collection: MolNFT v1 / PDB
Contract: 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
Owner: 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
Molecule: Insulin a chain (pH 7); Insulin B chain (pH 7)
UniProt: P01317
Ligands: DCE

Structure

x-ray diffraction · 2 distinct chains · 55% helix, 4% strand (DSSP).

Chain	Molecule	Type	Residues	Secondary structure	UniProt
A	Insulin a chain (pH 7)	protein	21	67% helix, 5% strand	P01317
B	Insulin B chain (pH 7)	protein	30	47% helix, 3% strand	P01317

Ligands and binding sites

DCE · 1,2-dichloroethane · ligand · chains B

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Interfaces

1 interface between chains.

A–B · 794 Å² buried · 9 hydrogen bonds · protein-protein

Modifications

Observed in the structure: 3 disulfide (disulfide).

Compared structures

1APH · Conformational changes in cubic insulin crystals in the pH range 7-11 · the same entry in another collection · chain A against A, TM-score 1.00
P01317 · AlphaFold monomer V2.0 prediction for insulin (P01317) · a predicted model of the same protein · chain B against A, TM-score 0.42

Analyse

Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
Every structure of UniProt P01317
Elsewhere: RCSB PDB 1APH · PDBe 1APH · UniProt P01317

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