Blue fluorescent protein MKALAMA1

Structure

x-ray diffraction · 1 distinct chain · 11% helix, 50% strand (DSSP).

Ligands and binding sites

• GYS · [(4Z)-2-(1-amino-2-hydroxyethyl)-4-(4-hydroxybenzylidene)-5-oxo-4,5-dihydro-1H-imidazol-1-yl]acetic acid · ligand · 2 copies · chains A, B
• SO4 · Sulfate ion · ion · 6 copies · chains A, B
• CL · Chloride ion · ion · 4 copies · chains A, B
• MES · 2-(N-morpholino)-ethanesulfonic acid · additive · 2 copies · chains A

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Pockets

4 pockets found on a 1 Å grid, 3 with a druggability of 0.5 or more.

Interfaces

1 interface between chains.

• A–B · 778 Ų buried · 9 hydrogen bonds · protein-protein

Modifications

Observed in the structure: 4 cofactor, 2 modified (chromophore, oxidation).

Compared structures

• 4ORN · Blue Fluorescent Protein mKalama1 · the same entry in another collection · chain A against A, TM-score 1.00
• P42212 · AlphaFold monomer V2.0 prediction for green fluorescent protein (P42212) · a predicted model of the same protein · chain A against A, TM-score 0.99

Analyse

• Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
• Every structure of UniProt P42212
• Elsewhere: RCSB PDB 4ORN · PDBe 4ORN · UniProt P42212