Research workbench
Structure of the replicating complex of a pol alpha family DNA polymerase
Analyse Structure of the replicating complex of a pol alpha family DNA polymerase in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 1IG9
- Type
- protein + DNA
- Length
- 933 residues
- Chains
- 3
- Method
- x-ray diffraction
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- 5'-D(*AP*CP*AP*GP*GP*TP*AP*AP*GP*CP*AP*GP*TP*CP*CP*GP*CP*G) -3'…
- EC
- 2.7.7.7
- UniProt
- Q38087
- Ligands
- TTP, CA
- Pockets
- 11 · best druggability 0.84
Structure
x-ray diffraction · 3 distinct chains · 43% helix, 18% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| T | 5'-D(*AP*CP*AP*GP*GP*TP*AP*AP*GP*CP*AP*GP*TP*CP*CP*GP*CP*G) -3' | dna | 18 | — | — |
| P | 5'-D(*GP*CP*GP*GP*AP*CP*TP*GP*CP*TP*TP*AP*CP*(DOC))-3' | dna | 14 | — | — |
| A | DNA polymerase (EC 2.7.7.7) | protein | 901 | 43% helix, 18% strand | Q38087 |
Ligands and binding sites
- TTP · Thymidine-5'-triphosphate · ligand · chains T, P, A
- CA · Calcium ion · metal · 4 copies · chains A, P
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
11 pockets found on a 1 Å grid, 6 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 339 | 0.84 | 0.94 | 29 | empty |
| 2 | 821 | 0.77 | 0.81 | 79 | CA |
| 3 | 884 | 0.77 | 0.80 | 73 | CA, TTP |
| 4 | 316 | 0.61 | 0.78 | 45 | empty |
| 5 | 121 | 0.57 | 0.84 | 20 | empty |
Interfaces
3 interfaces between chains.
- T–A · 1112 Ų buried · 15 hydrogen bonds · protein-nucleic
- P–A · 705 Ų buried · 15 hydrogen bonds · protein-nucleic
- T–P · 637 Ų buried · 68 hydrogen bonds · nucleic-nucleic
Modifications
Observed in the structure: 1 modified (nucleotide modification).
Compared structures
- 1IG9 · Structure of the Replicating Complex of a Pol Alpha Family DNA Polymerase · the same entry in another collection · chain T against T, TM-score 1.00
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt Q38087
- Elsewhere: RCSB PDB 1IG9 · PDBe 1IG9 · UniProt Q38087
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