Research workbench
1.55A Resolution Structure of NanoLuc Luciferase
Analyse 1.55A Resolution Structure of NanoLuc Luciferase in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 7SNS
- Type
- protein
- Length
- 685 residues
- Chains
- 4
- Method
- x-ray diffraction
- Collection
- MolNFT v2 / PDB Bank
- Contract
- 0xd58B01f6C18086e5202cdC5D7Ad3E41790360102
- Owner
- 0xF4e1AA6f26B7DA48EeAD9B24a97927303eeA07b8
- Resolution
- 1.55
- Source
- Oplophorus gracilirostris
- Molecule
- Oplophorus-luciferin 2-monooxygenase catalytic subunit
- EC
- 1.13.12.13
- UniProt
- Q9GV45
- Pockets
- 8 · best druggability 0.83
Structure
x-ray diffraction · resolution 1.55 · 3 distinct chains · 15% helix, 53% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | Oplophorus-luciferin 2-monooxygenase catalytic subunit (EC 1.13.12.13) | protein | 171 | 16% helix, 52% strand | Q9GV45 |
| B | Oplophorus-luciferin 2-monooxygenase catalytic subunit (EC 1.13.12.13) | protein | 170 | 13% helix, 53% strand | Q9GV45 |
| C, D | Oplophorus-luciferin 2-monooxygenase catalytic subunit (EC 1.13.12.13) | protein | 172 | 16% helix, 53% strand | Q9GV45 |
Ligands and binding sites
- ACT · Acetate ion · additive · 20 copies · chains A, B, C, D
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
8 pockets found on a 1 Å grid, 6 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 697 | 0.83 | 0.87 | 88 | ACT |
| 2 | 289 | 0.82 | 0.93 | 51 | ACT |
| 3 | 340 | 0.81 | 0.91 | 40 | empty |
| 4 | 144 | 0.78 | 0.95 | 36 | ACT |
| 5 | 91 | 0.75 | 0.96 | 22 | ACT |
Interfaces
6 interfaces between chains.
- A–C · 1265 Ų buried · 13 hydrogen bonds · protein-protein
- B–D · 1162 Ų buried · 11 hydrogen bonds · protein-protein
- A–B · 987 Ų buried · 6 hydrogen bonds · protein-protein
- C–D · 902 Ų buried · 7 hydrogen bonds · protein-protein
- A–D · 295 Ų buried · 5 hydrogen bonds · protein-protein
Compared structures
- Q9GV45 · AlphaFold monomer V2.0 prediction for oplophorus-luciferin 2-monooxygenase catalytic… · a predicted model of the same protein · chain B against A, TM-score 0.97
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt Q9GV45
- Elsewhere: RCSB PDB 7SNS · PDBe 7SNS · UniProt Q9GV45
GL1X is a live explorer of the GenesisL1 blockchain. Turn on JavaScript for search, charts and interactive views.