Research workbench
Cryo-EM structure of the folate-specific ECF transporter complex in DDM micelles
Analyse Cryo-EM structure of the folate-specific ECF transporter complex in DDM micelles in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 7NNT
- Type
- protein
- Length
- 979 residues
- Chains
- 4
- Method
- electron microscopy
- Collection
- MolNFT v2 / PDB Bank
- Contract
- 0xd58B01f6C18086e5202cdC5D7Ad3E41790360102
- Owner
- 0xF4e1AA6f26B7DA48EeAD9B24a97927303eeA07b8
- Resolution
- 3.4
- Source
- Lactobacillus delbrueckii subsp. bulgaricus (strain ATCC 11842 / DSM 20081 /…
- Molecule
- Energy-coupling factor transporter ATP-binding protein EcfA1; Energy-coupling factor…
- EC
- 7.-.-.-, 3.6.3.-
- UniProt
- Q1GBJ0, Q1GBI9, Q1G929, Q1GBI8
- Pockets
- 10 · best druggability 0.84
Structure
electron microscopy · resolution 3.4 · 4 distinct chains · 56% helix, 12% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | Energy-coupling factor transporter ATP-binding protein EcfA1 (EC 7.-.-.-) | protein | 279 | 44% helix, 19% strand | Q1GBJ0 |
| B | Energy-coupling factor transporter ATP-binding protein EcfA2 (EC 3.6.3.-) | protein | 272 | 41% helix, 21% strand | Q1GBI9 |
| C | Folate family ECF transporter S component | protein | 169 | 75% helix, 2% strand | Q1G929 |
| D | Energy-coupling factor transporter transmembrane protein EcfT | protein | 259 | 73% helix, 0% strand | Q1GBI8 |
Pockets
10 pockets found on a 1 Å grid, 6 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 1164 | 0.84 | 0.85 | 119 | empty |
| 2 | 943 | 0.79 | 0.81 | 64 | empty |
| 3 | 214 | 0.71 | 0.88 | 31 | empty |
| 4 | 251 | 0.60 | 0.79 | 29 | empty |
| 5 | 181 | 0.60 | 0.82 | 26 | empty |
Interfaces
6 interfaces between chains.
- C–D · 2895 Ų buried · 2 hydrogen bonds · protein-protein
- A–D · 1472 Ų buried · 22 hydrogen bonds · protein-protein
- A–B · 1341 Ų buried · 2 hydrogen bonds · protein-protein
- B–D · 820 Ų buried · 9 hydrogen bonds · protein-protein
- B–C · 232 Ų buried · 2 hydrogen bonds · protein-protein
Compared structures
- 7NNT · Cryo-EM structure of the folate-specific ecf transporter complex in ddm micelles · the same entry in another collection · chain A against A, TM-score 1.00
- Q1GBI9 · AlphaFold monomer V2.0 prediction for energy-coupling factor transporter ATP-binding… · a predicted model of the same protein · chain B against A, TM-score 0.99
- Q1GBJ0 · AlphaFold monomer V2.0 prediction for energy-coupling factor transporter ATP-binding… · a predicted model of the same protein · chain A against A, TM-score 0.97
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt Q1GBJ0
- Every structure of UniProt Q1GBI9
- Every structure of UniProt Q1G929
- Every structure of UniProt Q1GBI8
- Elsewhere: RCSB PDB 7NNT · PDBe 7NNT · UniProt Q1GBJ0 · UniProt Q1GBI9 · UniProt Q1G929 · UniProt Q1GBI8
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