Research workbench
Intermediate engineered variant of I-OnuI meganuclease with improved thermostability and partially altered specificity
Analyse Intermediate engineered variant of I-OnuI meganuclease with improved… in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 6UWH
- Type
- protein + DNA
- Length
- 346 residues
- Chains
- 3
- Method
- x-ray diffraction
- Collection
- MolNFT v2 / PDB Bank
- Contract
- 0xd58B01f6C18086e5202cdC5D7Ad3E41790360102
- Owner
- 0xF4e1AA6f26B7DA48EeAD9B24a97927303eeA07b8
- Resolution
- 2.299
- Source
- synthetic construct; SYNTHETIC CONSTRUCT
- Molecule
- I-OnuI-e-Therm-hChr11v1; DNA (26-mer)
- Ligands
- CA
- Pockets
- 7 · best druggability 0.74
Structure
x-ray diffraction · resolution 2.299 · 3 distinct chains · 49% helix, 23% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | I-OnuI-e-Therm-hChr11v1 | protein | 294 | 49% helix, 23% strand | — |
| B | DNA (26-mer) | dna | 26 | — | — |
| C | DNA (26-mer) | dna | 26 | — | — |
Ligands and binding sites
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
7 pockets found on a 1 Å grid, 1 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 115 | 0.74 | 0.95 | 26 | CA |
| 2 | 80 | 0.50 | 0.84 | 21 | empty |
| 3 | 70 | 0.44 | 0.82 | 19 | empty |
| 4 | 77 | 0.39 | 0.78 | 12 | empty |
| 5 | 68 | 0.30 | 0.75 | 13 | empty |
Interfaces
3 interfaces between chains.
- A–B · 1656 Ų buried · 20 hydrogen bonds · protein-nucleic
- A–C · 1426 Ų buried · 32 hydrogen bonds · protein-nucleic
- B–C · 1259 Ų buried · 100 hydrogen bonds · nucleic-nucleic
Compared structures
- 6UWH · Intermediate engineered variant of I-onui meganuclease with improved thermostability and… · the same entry in another collection · chain A against A, TM-score 1.00
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Elsewhere: RCSB PDB 6UWH · PDBe 6UWH
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