Research workbench
CryoEM structure of the MDA5-dsRNA filament with 93 degree twist and without nucleotide
Analyse CryoEM structure of the MDA5-dsRNA filament with 93 degree twist and without… in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 6H66
- Type
- protein + RNA
- Length
- 697 residues
- Chains
- 3
- Method
- electron microscopy
- Collection
- MolNFT v2 / PDB Bank
- Contract
- 0xd58B01f6C18086e5202cdC5D7Ad3E41790360102
- Owner
- 0xF4e1AA6f26B7DA48EeAD9B24a97927303eeA07b8
- Resolution
- 4.16
- Source
- Mus musculus; SYNTHETIC CONSTRUCT
- Molecule
- Interferon-induced helicase C domain-containing protein 1; RNA…
- EC
- 3.6.4.13
- UniProt
- Q8R5F7
- Ligands
- ZN
- Pockets
- 14 · best druggability 0.86
Structure
electron microscopy · resolution 4.16 · 3 distinct chains · 48% helix, 11% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | Interferon-induced helicase C domain-containing protein 1 (EC 3.6.4.13) | protein | 667 | 48% helix, 11% strand | Q8R5F7 |
| X | RNA (5'-R(P*UP*CP*CP*AP*UP*GP*CP*GP*CP*AP*UP*GP*AP*CP*G)-3') | rna | 15 | — | — |
| Y | RNA (5'-R(P*CP*GP*UP*CP*AP*UP*GP*CP*GP*CP*AP*UP*GP*GP*A)-3') | rna | 15 | — | — |
Ligands and binding sites
- ZN · Zinc ion · metal · chains A
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
14 pockets found on a 1 Å grid, 7 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 1106 | 0.86 | 0.88 | 116 | empty |
| 2 | 347 | 0.72 | 0.84 | 51 | empty |
| 3 | 313 | 0.72 | 0.85 | 24 | empty |
| 4 | 276 | 0.70 | 0.85 | 42 | empty |
| 5 | 118 | 0.68 | 0.90 | 24 | empty |
Interfaces
3 interfaces between chains.
- A–Y · 1008 Ų buried · 19 hydrogen bonds · protein-nucleic
- A–X · 974 Ų buried · 13 hydrogen bonds · protein-nucleic
- X–Y · 722 Ų buried · 62 hydrogen bonds · nucleic-nucleic
Compared structures
- 6H66 · Cryoem structure of the MDA5-dsrna filament with 93 degree twist and without nucleotide · the same entry in another collection · chain A against A, TM-score 1.00
- Q8R5F7 · AlphaFold monomer V2.0 prediction for interferon-induced helicase C domain-containing… · a predicted model of the same protein · chain A against A, TM-score 0.97
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt Q8R5F7
- Elsewhere: RCSB PDB 6H66 · PDBe 6H66 · UniProt Q8R5F7
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