Research workbench
Human DNA polymerase lambda L431A mutant- MgdCTP binary and complex with 6 paired DNA
Analyse Human DNA polymerase lambda L431A mutant- MgdCTP binary and complex with 6… in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 5CR0
- Type
- protein + DNA
- Length
- 501 residues
- Chains
- 4
- Method
- x-ray diffraction
- Collection
- MolNFT v2 / PDB Bank
- Contract
- 0xd58B01f6C18086e5202cdC5D7Ad3E41790360102
- Owner
- 0xF4e1AA6f26B7DA48EeAD9B24a97927303eeA07b8
- Resolution
- 2.75
- Source
- Homo sapiens; SYNTHETIC CONSTRUCT
- Molecule
- DNA polymerase lambda; DNA (5'-D(*CP*AP*GP*TP*AP*C)-3'); DNA (5'-D(P*GP*TP*AP*CP*TP*G)-3')
- EC
- 2.7.7.7
- UniProt
- Q9UGP5
- Ligands
- DCP, MG
- Pockets
- 4 · best druggability 0.71
Structure
x-ray diffraction · resolution 2.75 · 4 distinct chains · 41% helix, 21% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | DNA polymerase lambda (EC 2.7.7.7) | protein | 246 | 43% helix, 21% strand | Q9UGP5 |
| B | DNA polymerase lambda (EC 2.7.7.7) | protein | 243 | 40% helix, 22% strand | Q9UGP5 |
| P | DNA (5'-D(*CP*AP*GP*TP*AP*C)-3') | dna | 6 | — | — |
| T | DNA (5'-D(P*GP*TP*AP*CP*TP*G)-3') | dna | 6 | — | — |
Ligands and binding sites
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
4 pockets found on a 1 Å grid, 2 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 159 | 0.71 | 0.90 | 26 | empty |
| 2 | 655 | 0.67 | 0.76 | 62 | empty |
| 3 | 68 | 0.47 | 0.83 | 17 | empty |
| 4 | 120 | 0.39 | 0.74 | 25 | DCP |
Interfaces
4 interfaces between chains.
- A–T · 582 Ų buried · 5 hydrogen bonds · protein-nucleic
- A–B · 532 Ų buried · 4 hydrogen bonds · protein-protein
- A–P · 520 Ų buried · 17 hydrogen bonds · protein-nucleic
- P–T · 287 Ų buried · 26 hydrogen bonds · nucleic-nucleic
Compared structures
- 5CR0 · Human DNA polymerase lambda L431A mutant- mgdctp binary and complex with 6 paired DNA · the same entry in another collection · chain A against A, TM-score 1.00
- Q9UGP5 · AlphaFold monomer V2.0 prediction for DNA polymerase lambda (Q9UGP5) · a predicted model of the same protein · chain A against A, TM-score 0.98
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt Q9UGP5
- Elsewhere: RCSB PDB 5CR0 · PDBe 5CR0 · UniProt Q9UGP5
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