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Structure of H200N variant of Homoprotocatechuate 2,3-Dioxygenase from B.fuscum in complex with HPCA at 1.57 Ang resolution
Analyse Structure of H200N variant of Homoprotocatechuate 2,3-Dioxygenase from B.fuscum… in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 4Z6Q
- Type
- protein
- Length
- 1,434 residues
- Chains
- 4
- Method
- x-ray diffraction
- Collection
- MolNFT v2 / PDB Bank
- Contract
- 0xd58B01f6C18086e5202cdC5D7Ad3E41790360102
- Owner
- 0xF4e1AA6f26B7DA48EeAD9B24a97927303eeA07b8
- Resolution
- 1.57
- Source
- Brevibacterium fuscum
- Molecule
- Homoprotocatechuate 2,3-dioxygenase
- UniProt
- Q45135
- Ligands
- DHY, FE2, CA, CL
- Pockets
- 20 · best druggability 0.84
Structure
x-ray diffraction · resolution 1.57 · 4 distinct chains · 17% helix, 35% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | Homoprotocatechuate 2,3-dioxygenase | protein | 358 | 17% helix, 35% strand | Q45135 |
| B | Homoprotocatechuate 2,3-dioxygenase | protein | 360 | 18% helix, 35% strand | Q45135 |
| C | Homoprotocatechuate 2,3-dioxygenase | protein | 357 | 17% helix, 35% strand | Q45135 |
| D | Homoprotocatechuate 2,3-dioxygenase | protein | 359 | 17% helix, 35% strand | Q45135 |
Ligands and binding sites
- DHY · 2-(3,4-dihydroxyphenyl)acetic acid · ligand · 2 copies · chains C, D
- FE2 · Fe (II) ion · metal · 4 copies · chains A, B, C, D
- CA · Calcium ion · metal · chains B
- CL · Chloride ion · ion · 2 copies · chains A, B
- P6G · Hexaethylene glycol · additive · 5 copies · chains A, B, C, D
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
20 pockets found on a 1 Å grid, 8 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 369 | 0.84 | 0.94 | 46 | empty |
| 2 | 339 | 0.84 | 0.94 | 45 | empty |
| 3 | 131 | 0.79 | 0.97 | 25 | empty |
| 4 | 102 | 0.78 | 0.98 | 24 | empty |
| 5 | 61 | 0.73 | 0.99 | 28 | empty |
Interfaces
6 interfaces between chains.
- B–D · 1724 Ų buried · 44 hydrogen bonds · protein-protein
- C–D · 1654 Ų buried · 38 hydrogen bonds · protein-protein
- A–B · 1643 Ų buried · 35 hydrogen bonds · protein-protein
- A–C · 1596 Ų buried · 43 hydrogen bonds · protein-protein
- B–C · 530 Ų buried · 3 hydrogen bonds · protein-protein
Compared structures
- 4Z6Q · Structure of H200N variant of homoprotocatechuate 2,3-dioxygenase from B.FUSCUM in… · the same entry in another collection · chain A against A, TM-score 1.00
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt Q45135
- Elsewhere: RCSB PDB 4Z6Q · PDBe 4Z6Q · UniProt Q45135
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