Insulin fibrillation is the janus face of induced fit. a chiaral clamp stabilizes the native state at the expense of activity

Structure

x-ray diffraction · 2 distinct chains · 49% helix, 8% strand (DSSP).

Ligands and binding sites

• IPH · Phenol · ligand · chains C, D
• ZN · Zinc ion · metal · 2 copies · chains B, D
• CL · Chloride ion · ion · 2 copies · chains B, D

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Interfaces

5 interfaces between chains.

• A–B · 828 Ų buried · 10 hydrogen bonds · protein-protein
• C–D · 622 Ų buried · 4 hydrogen bonds · protein-protein
• B–D · 613 Ų buried · 10 hydrogen bonds · protein-protein
• A–D · 70 Ų buried · 1 hydrogen bond · protein-protein
• B–C · 22 Ų buried · 0 hydrogen bonds · protein-protein

Modifications

Observed in the structure: 6 disulfide, 2 d-amino (disulfide, D-amino acid).

Compared structures

• 3P2X · Insulin fibrillation is the Janus face of induced fit. A chiaral clamp stabilizes the… · the same entry in another collection · chain A against A, TM-score 1.00

Analyse

• Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
• Every structure of UniProt P01308
• Elsewhere: RCSB PDB 3P2X · PDBe 3P2X · UniProt P01308