MolNFT v1 · #6170 · protein

E. COLI (lacz) beta-galactosidase (orthorhombic)

1F4H: “E. COLI (lacz) beta-galactosidase (orthorhombic)”. X-ray diffraction, 1 ligand (MG), 20 pockets (best druggability 0.81). MolNFT v1 #6170 on GenesisL1: 3D structure, binding sites and pockets in GL1X.

PDB: 1F4H
Type: protein
Length: 4,084 residues
Chains: 4
Method: x-ray diffraction
Collection: MolNFT v1 / PDB
Contract: 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
Owner: 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
Molecule: Beta-galactosidase
EC: 3.2.1.23
UniProt: P00722
Ligands: MG
Pockets: 20 · best druggability 0.81

Structure

x-ray diffraction · 1 distinct chain · 14% helix, 39% strand (DSSP).

Chain	Molecule	Type	Residues	Secondary structure	UniProt
A, B, C, D	Beta-galactosidase (EC 3.2.1.23)	protein	1021	14% helix, 39% strand	P00722

Ligands and binding sites

MG · Magnesium ion · metal · 8 copies · chains A, B, C, D

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Pockets

20 pockets found on a 1 Å grid, 20 with a druggability of 0.5 or more.

Pocket	Volume (Å³)	Druggability	Buriedness	Lining residues	Inside
1	932	0.81	0.83	89	empty
2	825	0.81	0.84	113	empty
3	713	0.80	0.84	88	empty
4	704	0.78	0.83	78	empty
5	79	0.76	0.99	22	empty

Interfaces

6 interfaces between chains.

A–D · 2414 Å² buried · 27 hydrogen bonds · protein-protein
B–C · 2366 Å² buried · 26 hydrogen bonds · protein-protein
A–B · 2068 Å² buried · 28 hydrogen bonds · protein-protein
C–D · 1972 Å² buried · 24 hydrogen bonds · protein-protein
B–D · 101 Å² buried · 0 hydrogen bonds · protein-protein

Compared structures

1F4H · E. COLI (lacz) beta-galactosidase (orthorhombic) · the same entry in another collection · chain D against D, TM-score 1.00
P00722 · AlphaFold monomer V2.0 prediction for beta-galactosidase (P00722) · a predicted model of the same protein · chain C against A, TM-score 1.00

Analyse

Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
Every structure of UniProt P00722
Elsewhere: RCSB PDB 1F4H · PDBe 1F4H · UniProt P00722

GL1X is a live explorer of the GenesisL1 blockchain. Turn on JavaScript for search, charts and interactive views.