MolNFT v1 · #46543 · protein
The C-terminal loop of the homing endonuclease I-crei is essential for DNA binding and cleavage. identification of a novel site for specificity engineering in the I-crei scaffold
2O7M · DNA endonuclease I-crei: “The C-terminal loop of the homing endonuclease I-crei is essential for DNA binding and cleavage.…”. X-ray diffraction, 6 pockets (best druggability 0.43). MolNFT v1 #46543 on GenesisL1: 3D structure…
- PDB
- 2O7M
- Type
- protein
- Length
- 306 residues
- Chains
- 2
- Method
- x-ray diffraction
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- DNA endonuclease I-crei
- EC
- 3.1.-.-
- UniProt
- P05725
- Pockets
- 6 · best druggability 0.43
Structure
x-ray diffraction · 1 distinct chain · 56% helix, 20% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A, B | DNA endonuclease I-crei (EC 3.1.-.-) | protein | 153 | 56% helix, 20% strand | P05725 |
Pockets
6 pockets found on a 1 Å grid, 0 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 157 | 0.43 | 0.74 | 25 | empty |
| 2 | 160 | 0.42 | 0.74 | 24 | empty |
| 3 | 148 | 0.40 | 0.73 | 22 | empty |
| 4 | 92 | 0.39 | 0.76 | 15 | empty |
| 5 | 74 | 0.39 | 0.78 | 13 | empty |
Interfaces
1 interface between chains.
- A–B · 971 Ų buried · 10 hydrogen bonds · protein-protein
Compared structures
- 2O7M · The C-terminal loop of the homing endonuclease I-CreI is essential for DNA binding and… · the same entry in another collection · chain A against A, TM-score 1.00
- P05725 · AlphaFold monomer V2.0 prediction for DNA endonuclease I-crei (P05725) · a predicted model of the same protein · chain A against A, TM-score 0.90
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P05725
- Elsewhere: RCSB PDB 2O7M · PDBe 2O7M · UniProt P05725
GL1X is a live explorer of the GenesisL1 blockchain. Turn on JavaScript for search, charts and interactive views.