MolNFT v1 · #32511 · protein

Crystal structure of the TIM9 TIM10 hexameric complex

2BSK · Mitochondrial import inner membrane translocase subunit…: “Crystal structure of the TIM9 TIM10 hexameric complex”. X-ray diffraction, 9 pockets (best druggability 0.47). MolNFT v1 #32511 on GenesisL1: 3D structure, binding sites and…

PDB: 2BSK
Type: protein
Length: 434 residues
Chains: 6
Method: x-ray diffraction
Collection: MolNFT v1 / PDB
Contract: 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
Owner: 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
Molecule: Mitochondrial import inner membrane translocase subunit TIM9 a; Mitochondrial import…
UniProt: Q9Y5J7, P62072
Pockets: 9 · best druggability 0.47

Structure

x-ray diffraction · 6 distinct chains · 71% helix, 0% strand (DSSP).

Chain	Molecule	Type	Residues	Secondary structure	UniProt
A	Mitochondrial import inner membrane translocase subunit TIM9 a	protein	73	77% helix, 0% strand	Q9Y5J7
B	Mitochondrial import inner membrane translocase subunit TIM10	protein	65	69% helix, 0% strand	P62072
C	Mitochondrial import inner membrane translocase subunit TIM9 a	protein	79	61% helix, 0% strand	Q9Y5J7
D	Mitochondrial import inner membrane translocase subunit TIM10	protein	90	71% helix, 0% strand	P62072
E	Mitochondrial import inner membrane translocase subunit TIM9 a	protein	63	76% helix, 0% strand	Q9Y5J7
F	Mitochondrial import inner membrane translocase subunit TIM10	protein	64	77% helix, 0% strand	P62072

Pockets

9 pockets found on a 1 Å grid, 0 with a druggability of 0.5 or more.

Pocket	Volume (Å³)	Druggability	Buriedness	Lining residues	Inside
1	188	0.47	0.75	29	empty
2	140	0.40	0.74	25	empty
3	128	0.39	0.74	18	empty
4	90	0.38	0.76	19	empty
5	79	0.36	0.76	13	empty

Interfaces

9 interfaces between chains.

B–E · 1372 Å² buried · 9 hydrogen bonds · protein-protein
C–F · 1313 Å² buried · 9 hydrogen bonds · protein-protein
A–D · 1249 Å² buried · 12 hydrogen bonds · protein-protein
A–B · 1075 Å² buried · 12 hydrogen bonds · protein-protein
E–F · 1038 Å² buried · 9 hydrogen bonds · protein-protein

Modifications

Observed in the structure: 19 modified, 12 disulfide (engineered, disulfide).

Compared structures

2BSK · Crystal structure of the TIM9 Tim10 hexameric complex · the same entry in another collection · chain F against F, TM-score 1.00
P62072 · AlphaFold monomer V2.0 prediction for mitochondrial import inner membrane translocase… · a predicted model of the same protein · chain F against A, TM-score 0.94
Q9Y5J7 · AlphaFold monomer V2.0 prediction for mitochondrial import inner membrane translocase… · a predicted model of the same protein · chain A against A, TM-score 0.93

Analyse

Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
Every structure of UniProt Q9Y5J7
Every structure of UniProt P62072
Elsewhere: RCSB PDB 2BSK · PDBe 2BSK · UniProt Q9Y5J7 · UniProt P62072

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