Diabetes-associated mutations in human insulin: crystal structure and photo-cross-linking studies of a-chain variant insulin wakayama

Structure

x-ray diffraction · 2 distinct chains · 63% helix, 8% strand (DSSP).

Ligands and binding sites

• IPH · Phenol · ligand · 2 copies · chains A, B, C, D
• ZN · Zinc ion · metal · 2 copies · chains B, D
• CL · Chloride ion · ion · 2 copies · chains B, D

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Interfaces

5 interfaces between chains.

• B–D · 686 Ų buried · 11 hydrogen bonds · protein-protein
• A–B · 686 Ų buried · 7 hydrogen bonds · protein-protein
• C–D · 674 Ų buried · 6 hydrogen bonds · protein-protein
• B–C · 72 Ų buried · 1 hydrogen bond · protein-protein
• A–D · 30 Ų buried · 0 hydrogen bonds · protein-protein

Modifications

Observed in the structure: 6 disulfide (disulfide).

Compared structures

• 1XW7 · Diabetes-Associated Mutations in Human Insulin: Crystal Structure and Photo-Cross-Linking… · the same entry in another collection · chain A against A, TM-score 1.00

Analyse

• Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
• Every structure of UniProt P01308
• Elsewhere: RCSB PDB 1XW7 · PDBe 1XW7 · UniProt P01308