Solution structure of the yeast translation initiation factor EIF4E in complex with M7GDP and EIF4GI residues 393 to 490

Structure

solution nmr · 2 distinct chains · 28% helix, 13% strand (DSSP).

Ligands and binding sites

• MTN · S-[(1-oxyl-2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrol-3-yl)methyl] methanesulfonothioate · ligand · 4 copies · chains A, B
• M7G · 7N-methyl-8-hydroguanosine-5'-diphosphate · ligand · chains A

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Pockets

3 pockets found on a 1 Å grid, 1 with a druggability of 0.5 or more.

Interfaces

1 interface between chains.

• A–B · 2126 Ų buried · 21 hydrogen bonds · protein-protein

Modifications

Observed in the structure: 4 covalent (covalent ligand).

Compared structures

• 1RF8 · Solution structure of the yeast translation initiation factor eIF4E in complex with m7GDP… · the same entry in another collection · chain A against A, TM-score 1.00
• P07260 · AlphaFold monomer V2.0 prediction for eukaryotic translation initiation factor 4E (P07260) · a predicted model of the same protein · chain A against A, TM-score 0.76
• P39935 · AlphaFold monomer V2.0 prediction for eukaryotic initiation factor 4F subunit P150… · a predicted model of the same protein · chain B against A, TM-score 0.49

Analyse

• Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
• Every structure of UniProt P07260
• Every structure of UniProt P39935
• Elsewhere: RCSB PDB 1RF8 · PDBe 1RF8 · UniProt P07260 · UniProt P39935