Crystal structure of porcine cathepsin H determined at 2.1 ångström resolution: location of the mini-chain C-terminal carboxyl group defines cathepsin H aminopeptidase function

Structure

x-ray diffraction · 2 distinct chains · 29% helix, 23% strand (DSSP).

Ligands and binding sites

• NAG · 2-acetamido-2-deoxy-beta-D-glucopyranose · sugar · 2 copies · chains A, P
• BMA · beta-D-mannopyranose · sugar · chains A

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Interfaces

1 interface between chains.

• A–P · 473 Ų buried · 7 hydrogen bonds · protein-protein

Modifications

Observed in the structure: 4 disulfide, 1 glycan (disulfide, N-glycosylation).

Compared structures

• 8PCH · Crystal structure of porcine cathepsin H determined at 2.1 ångström resolution: location… · the same entry in another collection · chain A against A, TM-score 1.00
• O46427 · AlphaFold monomer V2.0 prediction for pro-cathepsin H (O46427) · a predicted model of the same protein · chain A against A, TM-score 0.99

Analyse

• Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
• Every structure of UniProt O46427
• Elsewhere: RCSB PDB 8PCH · PDBe 8PCH · UniProt O46427