MolNFT v1 · #191154 · protein
Cryo-EM structure of F-actin in the MG2+-ADP-pi nucleotide state.
8A2S · Actin, alpha skeletal muscle: “Cryo-EM structure of F-actin in the MG2+-ADP-pi nucleotide state.”. Electron microscopy, 3 ligands (ADP, MG, PO4), 20 pockets (best druggability 0.75). MolNFT v1 #191154 on GenesisL1: 3D structure…
- PDB
- 8A2S
- Type
- protein
- Length
- 1,835 residues
- Chains
- 5
- Method
- electron microscopy
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Actin, alpha skeletal muscle
- UniProt
- P68135
- Ligands
- ADP, MG, PO4
- Pockets
- 20 · best druggability 0.75
Structure
electron microscopy · 1 distinct chain · 45% helix, 21% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| C, A, B, D, E | Actin, alpha skeletal muscle | protein | 367 | 45% helix, 21% strand | P68135 |
Ligands and binding sites
- ADP · Adenosine-5'-diphosphate · ligand · 5 copies · chains C, A, B, D, E
- MG · Magnesium ion · metal · 5 copies · chains C, A, B, D, E
- PO4 · Phosphate ion · ion · 5 copies · chains C, A, B, D, E
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
20 pockets found on a 1 Å grid, 15 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 242 | 0.75 | 0.89 | 50 | ADP, MG, PO4 |
| 2 | 241 | 0.75 | 0.89 | 48 | ADP, MG, PO4 |
| 3 | 234 | 0.74 | 0.89 | 48 | ADP, MG, PO4 |
| 4 | 235 | 0.74 | 0.89 | 49 | ADP, MG, PO4 |
| 5 | 221 | 0.72 | 0.88 | 41 | ADP, MG, PO4 |
Interfaces
7 interfaces between chains.
- C–E · 1196 Ų buried · 13 hydrogen bonds · protein-protein
- B–D · 1192 Ų buried · 13 hydrogen bonds · protein-protein
- C–A · 1181 Ų buried · 13 hydrogen bonds · protein-protein
- A–B · 441 Ų buried · 6 hydrogen bonds · protein-protein
- C–B · 436 Ų buried · 6 hydrogen bonds · protein-protein
Modifications
Observed in the structure: 5 modified (methylation).
Compared structures
- 8A2S · Cryo-EM structure of F-actin in the Mg2+-ADP-Pi nucleotide state. · the same entry in another collection · chain E against E, TM-score 1.00
- P68135 · AlphaFold monomer V2.0 prediction for actin, alpha skeletal muscle (P68135) · a predicted model of the same protein · chain E against A, TM-score 0.96
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P68135
- Elsewhere: RCSB PDB 8A2S · PDBe 8A2S · UniProt P68135
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