MolNFT v1 · #188356 · protein
Complex gggn of AMPA-subtype iglur GLUA2 in complex with auxiliary subunit GAMMA2 (stargazin) at low glutamate concentration (20 um) in the presence of cyclothiazide (100 um)
7TNO · Isoform flip of glutamate receptor 2,VOLTAGE-dependent…: “Complex gggn of AMPA-subtype iglur GLUA2 in complex with auxiliary subunit GAMMA2 (stargazin) at low…”. Electron microscopy, 2 ligands (CYZ, GLU), 20 pockets (best…
- PDB
- 7TNO
- Type
- protein
- Length
- 2,402 residues
- Chains
- 4
- Method
- electron microscopy
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Isoform flip of glutamate receptor 2,VOLTAGE-dependent calcium channel gamma-3 subunit…
- UniProt
- P19491
- Ligands
- CYZ, GLU
- Pockets
- 20 · best druggability 0.82
Structure
electron microscopy · 3 distinct chains · 49% helix, 12% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A, C | Isoform flip of glutamate receptor 2,VOLTAGE-dependent calcium channel gamma-3 subunit chimera | protein | 601 | 49% helix, 11% strand | P19491 |
| B | Isoform flip of glutamate receptor 2,VOLTAGE-dependent calcium channel gamma-3 subunit chimera | protein | 600 | 48% helix, 12% strand | P19491 |
| D | Isoform flip of glutamate receptor 2,VOLTAGE-dependent calcium channel gamma-3 subunit chimera | protein | 600 | 49% helix, 12% strand | P19491 |
Ligands and binding sites
- CYZ · Cyclothiazide · ligand · 4 copies · chains A, D, B, C
- GLU · Glutamic acid · ligand · 3 copies · chains A, B, C
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
20 pockets found on a 1 Å grid, 17 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 896 | 0.82 | 0.84 | 101 | CYZ |
| 2 | 1081 | 0.81 | 0.82 | 96 | empty |
| 3 | 629 | 0.80 | 0.85 | 79 | CYZ |
| 4 | 901 | 0.74 | 0.78 | 81 | empty |
| 5 | 814 | 0.74 | 0.79 | 78 | empty |
Interfaces
4 interfaces between chains.
- B–C · 3563 Ų buried · 16 hydrogen bonds · protein-protein
- A–D · 3533 Ų buried · 18 hydrogen bonds · protein-protein
- A–B · 2905 Ų buried · 17 hydrogen bonds · protein-protein
- C–D · 2812 Ų buried · 17 hydrogen bonds · protein-protein
Modifications
Observed in the structure: 12 disulfide (disulfide).
Compared structures
- 7TNO · Complex GGGN of AMPA-subtype iGluR GluA2 in complex with auxiliary subunit gamma2… · the same entry in another collection · chain D against D, TM-score 1.00
- P19491 · AlphaFold monomer V2.0 prediction for glutamate receptor 2 (P19491) · a predicted model of the same protein · chain B against A, TM-score 0.65
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P19491
- Elsewhere: RCSB PDB 7TNO · PDBe 7TNO · UniProt P19491
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