MolNFT v1 · #188031 · protein

Octameric human twinkle helicase clinical variant W315L

7T8B · Twinkle mtdna helicase: “Octameric human twinkle helicase clinical variant W315L”. Electron microscopy, 20 pockets (best druggability 0.82). MolNFT v1 #188031 on GenesisL1: 3D structure, binding sites and pockets in GL1X.

PDB: 7T8B
Type: protein
Length: 4,131 residues
Chains: 8
Method: electron microscopy
Collection: MolNFT v1 / PDB
Contract: 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
Owner: 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
Molecule: Twinkle mtdna helicase
EC: 3.6.4.12
UniProt: Q96RR1
Pockets: 20 · best druggability 0.82

Structure

electron microscopy · 2 distinct chains · 37% helix, 22% strand (DSSP).

Chain	Molecule	Type	Residues	Secondary structure	UniProt
A	Twinkle mtdna helicase (EC 3.6.4.12)	protein	519	36% helix, 22% strand	Q96RR1
B, C, D, E, F, G, H	Twinkle mtdna helicase (EC 3.6.4.12)	protein	516	37% helix, 22% strand	Q96RR1

Pockets

20 pockets found on a 1 Å grid, 20 with a druggability of 0.5 or more.

Pocket	Volume (Å³)	Druggability	Buriedness	Lining residues	Inside
1	1036	0.82	0.83	90	empty
2	986	0.80	0.82	91	empty
3	935	0.80	0.82	87	empty
4	1047	0.80	0.81	96	empty
5	974	0.80	0.81	82	empty

Interfaces

8 interfaces between chains.

A–B · 1317 Å² buried · 10 hydrogen bonds · protein-protein
C–D · 1221 Å² buried · 7 hydrogen bonds · protein-protein
F–G · 1218 Å² buried · 7 hydrogen bonds · protein-protein
A–H · 1215 Å² buried · 7 hydrogen bonds · protein-protein
D–E · 1214 Å² buried · 7 hydrogen bonds · protein-protein

Compared structures

7T8B · Octameric Human Twinkle Helicase Clinical Variant W315L · the same entry in another collection · chain H against H, TM-score 1.00
Q96RR1 · AlphaFold monomer V2.0 prediction for twinkle protein, mitochondrial (Q96RR1) · a predicted model of the same protein · chain B against A, TM-score 0.97

Analyse

Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
Every structure of UniProt Q96RR1
Elsewhere: RCSB PDB 7T8B · PDBe 7T8B · UniProt Q96RR1

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