MolNFT v1 · #187152 · protein + RNA
CAS9:SGRNA (S. pyogenes) in the open-protein conformation
7S37 · CRISPR-associated endonuclease CAS9/CSN1: “CAS9:SGRNA (S. pyogenes) in the open-protein conformation”. Electron microscopy, 20 pockets (best druggability 0.82). MolNFT v1 #187152 on GenesisL1: 3D structure, binding sites and pockets…
- PDB
- 7S37
- Type
- protein + RNA
- Length
- 1,079 residues
- Chains
- 2
- Method
- electron microscopy
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- CRISPR-associated endonuclease CAS9/CSN1; Single-guide RNA
- EC
- 3.1.-.-
- UniProt
- Q99ZW2
- Pockets
- 20 · best druggability 0.82
Structure
electron microscopy · 2 distinct chains · 48% helix, 13% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| P | CRISPR-associated endonuclease CAS9/CSN1 (EC 3.1.-.-) | protein | 1033 | 48% helix, 13% strand | Q99ZW2 |
| R | Single-guide RNA | rna | 46 | — | — |
Pockets
20 pockets found on a 1 Å grid, 15 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 661 | 0.82 | 0.86 | 69 | empty |
| 2 | 417 | 0.78 | 0.87 | 47 | empty |
| 3 | 61 | 0.71 | 0.98 | 22 | empty |
| 4 | 310 | 0.70 | 0.84 | 33 | empty |
| 5 | 234 | 0.70 | 0.86 | 31 | empty |
Interfaces
1 interface between chains.
- P–R · 1790 Ų buried · 26 hydrogen bonds · protein-nucleic
Compared structures
- 7S37 · Cas9:sgRNA (S. pyogenes) in the open-protein conformation · the same entry in another collection · chain P against P, TM-score 1.00
- Q99ZW2 · AlphaFold monomer V2.0 prediction for CRISPR-associated endonuclease CAS9/CSN1 (Q99ZW2) · a predicted model of the same protein · chain P against A, TM-score 0.63
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt Q99ZW2
- Elsewhere: RCSB PDB 7S37 · PDBe 7S37 · UniProt Q99ZW2
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