MolNFT v1 · #182369 · protein
Cryo-EM structure of human HUWE1 in complex with DDIT4
7MOP · E3 ubiquitin-protein ligase HUWE1: “Cryo-EM structure of human HUWE1 in complex with DDIT4”. Electron microscopy, 20 pockets (best druggability 0.84). MolNFT v1 #182369 on GenesisL1: 3D structure, binding sites and pockets in GL1X.
- PDB
- 7MOP
- Type
- protein
- Length
- 2,452 residues
- Chains
- 2
- Method
- electron microscopy
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- E3 ubiquitin-protein ligase HUWE1; DNA damage-inducible transcript 4 protein
- EC
- 2.3.2.26
- UniProt
- Q7Z6Z7, Q9NX09
- Pockets
- 20 · best druggability 0.84
Structure
electron microscopy · 2 distinct chains · 65% helix, 5% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | E3 ubiquitin-protein ligase HUWE1 (EC 2.3.2.26) | protein | 2445 | 65% helix, 5% strand | Q7Z6Z7 |
| B | DNA damage-inducible transcript 4 protein | protein | 7 | 0% helix, 0% strand | Q9NX09 |
Pockets
20 pockets found on a 1 Å grid, 9 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 1167 | 0.84 | 0.85 | 125 | empty |
| 2 | 920 | 0.72 | 0.76 | 79 | empty |
| 3 | 404 | 0.68 | 0.80 | 51 | empty |
| 4 | 714 | 0.67 | 0.76 | 65 | empty |
| 5 | 359 | 0.67 | 0.81 | 35 | empty |
Interfaces
1 interface between chains.
- A–B · 511 Ų buried · 6 hydrogen bonds · protein-protein
Compared structures
- 7MOP · Cryo-EM structure of human HUWE1 in complex with DDIT4 · the same entry in another collection · chain B against B, TM-score 1.00
- Q9NX09 · AlphaFold monomer V2.0 prediction for DNA damage-inducible transcript 4 protein (Q9NX09) · a predicted model of the same protein · chain B against A, TM-score 0.59
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt Q7Z6Z7
- Every structure of UniProt Q9NX09
- Elsewhere: RCSB PDB 7MOP · PDBe 7MOP · UniProt Q7Z6Z7 · UniProt Q9NX09
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