MolNFT v1 · #180921 · protein
Trimeric human arginase 1 in complex with MAB4
7LF2 · Arginase-1: “Trimeric human arginase 1 in complex with MAB4”. Electron microscopy, 1 ligand (MN), 20 pockets (best druggability 0.84). MolNFT v1 #180921 on GenesisL1: 3D structure, binding sites and pockets in GL1X.
- PDB
- 7LF2
- Type
- protein
- Length
- 4,452 residues
- Chains
- 18
- Method
- electron microscopy
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Arginase-1; MAB4 monoclonal antibody heavy chain; MAB4 monoclonal antibody light chain
- EC
- 3.5.3.1
- UniProt
- P05089
- Ligands
- MN
- Pockets
- 20 · best druggability 0.84
Structure
electron microscopy · 3 distinct chains · 18% helix, 29% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A, B, C, J, Q, R | Arginase-1 (EC 3.5.3.1) | protein | 317 | 39% helix, 15% strand | P05089 |
| D, G, F, K, O, P | MAB4 monoclonal antibody heavy chain | protein | 211 | 0% helix, 36% strand | — |
| E, I, H, L, M, N | MAB4 monoclonal antibody light chain | protein | 214 | 6% helix, 41% strand | — |
Ligands and binding sites
- MN · Manganese (II) ion · metal · 12 copies · chains A, B, C, J, Q, R
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
20 pockets found on a 1 Å grid, 20 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 958 | 0.84 | 0.85 | 73 | empty |
| 2 | 811 | 0.83 | 0.86 | 61 | empty |
| 3 | 969 | 0.83 | 0.85 | 76 | empty |
| 4 | 1097 | 0.83 | 0.84 | 82 | empty |
| 5 | 1078 | 0.83 | 0.84 | 77 | empty |
Interfaces
58 interfaces between chains.
- P–N · 1890 Ų buried · 16 hydrogen bonds · protein-protein
- D–E · 1889 Ų buried · 15 hydrogen bonds · protein-protein
- F–H · 1888 Ų buried · 16 hydrogen bonds · protein-protein
- K–L · 1886 Ų buried · 15 hydrogen bonds · protein-protein
- O–M · 1869 Ų buried · 13 hydrogen bonds · protein-protein
Modifications
Observed in the structure: 24 disulfide (disulfide).
Compared structures
- 7LF2 · Trimeric human Arginase 1 in complex with mAb4 · the same entry in another collection · chain N against N, TM-score 1.00
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P05089
- Elsewhere: RCSB PDB 7LF2 · PDBe 7LF2 · UniProt P05089
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